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Simple visualization of submicroscopic protein clusters with a phase-separation-based fluorescent reporter.
Mumford TR, Rae D, Brackhahn E, Idris A, Gonzalez-Martinez D, Pal AA, Chung MC, Guan J, Rhoades E, Bugaj LJ
Cell Syst. 2024 Feb 21;15(2):166-179.e7. doi: 10.1016/j.cels.2024.01.005. Epub 2024 Feb 8. PubMed Article

Plasmids from Article

ID Plasmid Purpose
190024pHR_PGK_LaG17-mCherry-HOTag3Transiently expresses GFP-binding CluMPS (LaG17-mCh-HOTag3) in mammalian cells and lentiviral vector for constitutive expression.
190026pCMV Gab1(PRD)-mCherry-HOTag3Transiently expresses CluMPS for reporting on EML4-ALK oncogene clusters in mammalian cells.

Antibodies from Article