AspFlex: Molecular Tools to Study Gene Expression and Regulation in Acinetobacter baumannii.
Brychcy M, Kokodynski A, Lloyd D, Godoy VG
ACS Synth Biol. 2023 Aug 16. doi: 10.1021/acssynbio.3c00167. PubMed Article
Brychcy M, Kokodynski A, Lloyd D, Godoy VG
ACS Synth Biol. 2023 Aug 16. doi: 10.1021/acssynbio.3c00167. PubMed Article
A subset of these plasmids (#190114 to #190132) are available to purchase together in the AspFlex Kit (Kit #1000000217).
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 190114 | pMB1-A | Level 1 MoClo plasmid for A. baumannii |
| 190115 | pMB1-B | Level 1 MoClo plasmid for A. baumannii |
| 190116 | pMB1-C | Level 1 MoClo plasmid for A. baumannii |
| 190117 | pMB1-D | Level 1 MoClo plasmid for A. baumannii |
| 190118 | pMB1-D1 | Level 1 MoClo plasmid for A. baumannii |
| 190119 | pMB1-E | Level 1 MoClo plasmid for A. baumannii |
| 190120 | pMB2a | Level 2 MoClo plasmid for A. baumannii |
| 190121 | pMB2b | Level 2 MoClo plasmid for A. baumannii |
| 190122 | pMB2-A | Level 2 MoClo plasmid for A. baumannii |
| 190123 | pMB2-B | Level 2 MoClo plasmid for A. baumannii |
| 190124 | pMB2-C | Level 2 MoClo plasmid for A. baumannii |
| 190125 | pMB2-D | Level 2 MoClo plasmid for A. baumannii |
| 190126 | pMB3-A | Level 2 MoClo plasmid for A. baumannii |
| 190127 | pMB3-B | Level 2 MoClo plasmid for A. baumannii |
| 190128 | pBP_sgRNA | Level 0 MoClo plasmid for Golden Gate cloning of sgRNAs |
| 190129 | pMB1-A-ptet-dCas9 | Level 1 MoClo plasmid containing an aTC inducible dCas9 for CRISPRi |
| 190130 | pMB1-A-ptet-FLAG-dCas9 | Level 1 MoClo plasmid containing an aTC inducible FLAG tagged dCas9 for CRISPRi |
| 190131 | pBP_dCas9 | Level 0 MoClo plasmid containing dCas9 |
| 190132 | pMB1-A-pBAD-dCas9 | Level 1 MoClo plasmid containing an arabinose inducible dCas9 for CRISPRi |
| 204979 | pMB1-A-TelR | Level 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains |
| 204980 | pMB1-B-TelR | Level 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains |
| 204981 | pMB1-C-TelR | Level 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains |
| 204982 | pMB1-D-TelR | Level 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains |
| 204983 | pMB1-D1-TelR | Level 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains |
| 204984 | pMB1-E-TelR | Level 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains |
| 204985 | pMB2a-TelR | Level 2 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains |
| 204986 | pMB2b-TelR | Level 2 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains |
| 204987 | pMB2-A-TelR | Level 2 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains |
| 204988 | pMB2-B-TelR | Level 2 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains |
| 204989 | pMB2-C-TelR | Level 2 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains |
| 204990 | pMB2-D-TelR | Level 2 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains |
| 204991 | pMB3-A-TelR | Level 3 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains |
| 204992 | pMB3-B-TelR | Level 3 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains |