pBP_sgRNA
(Plasmid
#190128)
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Purpose(Empty Backbone) Level 0 MoClo plasmid for Golden Gate cloning of sgRNAs
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 190128 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBP / pSB1C3
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Backbone manufacturerPaul Freemont Lab / iGem
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Vector typeCRISPR
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Selectable markerslacZ
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBP_sgRNA was a gift from Veronica Godoy-Carter (Addgene plasmid # 190128 ; http://n2t.net/addgene:190128 ; RRID:Addgene_190128) -
For your References section:
AspFlex: Molecular Tools to Study Gene Expression and Regulation in Acinetobacter baumannii. Brychcy M, Kokodynski A, Lloyd D, Godoy VG. ACS Synth Biol. 2023 Aug 16. doi: 10.1021/acssynbio.3c00167. 10.1021/acssynbio.3c00167 PubMed 37587063
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