Optimized In Vitro CRISPR/Cas9 Gene Editing Tool in the West Nile Virus Mosquito Vector, Culex quinquefasciatus.
Torres TZB, Prince BC, Robison A, Ruckert C
Insects. 2022 Sep 19;13(9):856. doi: 10.3390/insects13090856. PubMed Article
Torres TZB, Prince BC, Robison A, Ruckert C
Insects. 2022 Sep 19;13(9):856. doi: 10.3390/insects13090856. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 190597 | pUb-Cas9-EGFP_cU6:6-sgRNA | The plasmid encodes S. pyogenes Cas9 and eGFP separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter. |
| 190598 | pUb-Cas9-mCherry_cU6:6-sgRNA | The plasmid encodes S. pyogenes Cas9 and mCherry separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter |