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Endogenous transcripts direct microRNA degradation in Drosophila, and this targeted degradation is required for proper embryonic development.
Kingston ER, Blodgett LW, Bartel DP
Mol Cell. 2022 Sep 15. pii: S1097-2765(22)00849-8. doi: 10.1016/j.molcel.2022.08.029.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
190608HA-doraWT_rescue_constructPuromycin-selectable expression of HA-tagged Dora (CG34401) in Drosophila S2 cells
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190609doraWT_rescue_constructPuromycin-selectable expression of Dora (CG34401) in Drosophila S2 cells
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190637dora[B]_rescue_constructPuromycin-selectable expression of Dora[T295M] (CG34401) in Drosophila S2 cells
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190638HA-dora[B]_rescue_constructPuromycin-selectable expression of HA-tagged Dora[T295M] (CG34401) in Drosophila S2 cells
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190639dora[A]_rescue_constructPuromycin-selectable expression of truncated Dora (CG34401) in Drosophila S2 cells
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190640HA-dora[A]_rescue_constructPuromycin-selectable expression of HA-tagged truncated Dora (CG34401) in Drosophila S2 cells
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190641HA-GFP_rescue_constructPuromycin-selectable expression of GFP in Drosophila S2 cells
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190700pAc-sgRNA-Cas9.dora_1Expresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cells
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190701pAc-sgRNA-Cas9.dora_2Expresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cells
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190702pAc-sgRNA-Cas9.dora_3Expresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cells
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190703pAc-sgRNA-Cas9.scr_1Expresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cells
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190704pAc-sgRNA-Cas9.scr_2Expresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cells
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190705pAc-sgRNA-Cas9.scr_3Expresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cells
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Recombinant Antibodies from Article