Addgene: Measuring G Protein Activation with Spectrally Resolved Fluorescence Fluctuation Spectroscopy

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Dave Piston
Foust et al

Measuring G Protein Activation with Spectrally Resolved Fluorescence Fluctuation Spectroscopy
Foust DJ, Piston DW
bioRxiv 2021.11.03.467169 Article

Plasmids from Article

ID	Plasmid	Purpose
190745	pCD86-mEGFP	Expression of mEGFP-tagged CD86 in mammalian cells.
190746	pCD86-mEYFP(Q69K)	Expression of mEYFP(Q69K)-tagged CD86 in mammalian cells.
190747	pCD86-mCherry2	Expression mCherry2-tagged CD86 in mammalian cells.
190748	pmCherry2-CD86-mEGFP	Expression of CD86 double tagged with mCherry2 (N-term) and mEGFP (C-term) in mammalian cells.
190749	pmCherry2-CD86-mEYFP(Q69K)	Expression of CD86 double tagged with mCherry2 (N-term) and mEYFP(Q69K) (C-term) in mammalian cells.
190750	pCD86-mEYFP(Q69K)-mEGFP	Expression of CD86 double tagged with mEYFP(Q69K) and mEGFP in mammalian cells.
190751	pmCherry2-CD86-mEYFP(Q69K)-mEGFP	Expression of triple tagged CD86 with mCherry2, mEYFP(Q69K), and mEGFP in mammalian cells.
190752	pmEGFP-DRD2	Expression of DRD2 with mEGFP tag on N-terminus.
190753	pmEGFP-ADRA2A	Expression of ADRA2A with mEGFP tag on N-terminus.
190755	pmCherry2-GNB1-T2A-mCherry2-GNG2-IRES-GNAI1-mEYFP(Q69K)	Expression of trimeric G protein with mCherry2 (GNB1 and GNG2) or mEYFP(Q69K) (GNAI1) tags.

Antibodies from Article