pmEGFP-ADRA2A
(Plasmid #190753)

• Purpose: Expression of ADRA2A with mEGFP tag on N-terminus.
• Depositing Lab: Dave Piston
• Depositing Organization: Washington University in Saint Louis
  Located in United States of America
• Publication: Foust et al Biophys J. 2025 Oct 21;124(20):3354-3365. doi: 10.1016/j.bpj.2024.08.008. Epub 2024 Aug 15.

Backbone

• Vector backbone: pcDNA3.1+/Hygro
• Backbone size w/o insert (bp): 5601
• Total vector size (bp): 8027
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ADRA2A
• Alt name: alpha-2A adrenergic receptor
• Alt name: alpha-2A AR
• Species: H. sapiens (human)
• GenBank ID: 150
• Entrez Gene: ADRA2A (a.k.a. ADRA2, ADRA2R, ADRAR, ALPHA2AAR, FPLD8)
• Promoter: CMV
• Tag / Fusion Protein:
  • mEGFP (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Addgene #24099 and #66216

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2021.11.03.467169v1 for bioRxiv preprint.

DNA (Catalog # 190753-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 190753-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.