Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Inducible CRISPR/Cas9 Allows for Multiplexed and Rapidly Segregated Single-Target Genome Editing in Synechocystis Sp. PCC 6803.
Cengic I, Canadas IC, Minton NP, Hudson EP
ACS Synth Biol. 2022 Aug 15. doi: 10.1021/acssynbio.2c00375.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
190790pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA[E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system.
190793pMD19-PBBa_J23117-sgRNA-SprTemplate vector to amplify single sgRNAs or pieces for multiplexing arrays. Has flanking BsaI-sites.
190794pMD19-Cas9_handle-S.pyogenes_terminator-PBBa_J23117-CmrTemplate vector to amplify pieces for creating multiplex sgRNA-arrays.

Antibodies from Article