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Inducible CRISPR/Cas9 Allows for Multiplexed and Rapidly Segregated Single-Target Genome Editing in Synechocystis Sp. PCC 6803.
Cengic I, Canadas IC, Minton NP, Hudson EP
ACS Synth Biol. 2022 Aug 15. doi: 10.1021/acssynbio.2c00375. PubMed Article

Plasmids from Article

ID Plasmid Purpose
190790pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA[E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system.
190793pMD19-PBBa_J23117-sgRNA-SprTemplate vector to amplify single sgRNAs or pieces for multiplexing arrays. Has flanking BsaI-sites.
190794pMD19-Cas9_handle-S.pyogenes_terminator-PBBa_J23117-CmrTemplate vector to amplify pieces for creating multiplex sgRNA-arrays.

Antibodies from Article