pMD19-Cas9_handle-S.pyogenes_terminator-PBBa_J23117-Cmr
(Plasmid #190794)

• Purpose: Template vector to amplify pieces for creating multiplex sgRNA-arrays.
• Depositing Lab: Paul Hudson
• Publication: Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.1021/acssynbio.2c00375.

Backbone

• Vector backbone: pMD19
• Backbone manufacturer: Takara
• Backbone size w/o insert (bp): 2125
• Total vector size (bp): 3987
• Modifications to backbone: A silent mutation of a BsaI-site found in the AmpR gene. A B0015 terminator and CmR cassette has been added to the backbone.
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: sgRNA-scaffold only
• gRNA/shRNA sequence: -
• Species: Synthetic
• Promoter: BBa_J23117

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI, SpeI (destroyed during cloning)
• 5′ sequencing primer: -
• 3′ sequencing primer: -

Resource Information

• Supplemental Documents:
  • pMD19-Cas9-handle-S.pyogenes-Term-PJ23117.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry