Purpose(Empty Backbone) sacB plasmid with polylinker; kanamycin/neomycin resistance: used as a conditionally lethal gene in Anabaena sp. strain PCC 7120 to select for double recombinants and to entrap insertion seq.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||70256||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonesee comments
- Backbone size (bp) 5941
Vector typeConditionally lethal
Selectable markersNeomycin (select with G418) ; for cyanobacteria
Growth in Bacteria
Growth instructionsWe consider it wise to store plasmids bearing a sacB gene at -80 deg. and to assess the very suspension that will be stored to be sure that it is highly sensitive to sucrose, and, similarly, to test individual colonies from which a sacB-bearing plasmid will be derived to be certain that sacB has retained great sensitivity to sucrose
Copy numberHigh Copy
- Cloning method Restriction Enzyme
A portion of this plasmid was derived from a plasmid made bysee comments
Terms and Licenses
sacB was derived from pUM24 (Ried, J. L., and A. Colimer Gene 57:239-246, 1987) and from pUCD800 (Gay, et al. 1985 J. Bacteriol. 164:918-921).
The neomycin/kanamycin determinant in pRL278 was derived from cassette C.K3 (Elhai and Wolk CP, 1988, Gene 68: 119-138).
The polylinker came, in large part, from the plC20R polylinker (Marsh et al.. 1984 Gene 32: 481-485) and from a polylinker region of plasmid pJRD184 (Heusterspreute et al.. 1985 Gene 37: 299-304).
Additional Reference for this plasmid:
Black TA, Cai Y, Wolk CP (1993) Spatial expression and autoregulation of hetR, a gene involved in the control of heterocyst development in Anabaena. Mol Microbiol 9: 77-84. Publisher’s correction (1993): Mol Microbiol 10: 1153
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRL278 was a gift from Peter Wolk (Addgene plasmid # 70256 ; http://n2t.net/addgene:70256 ; RRID:Addgene_70256)
For your References section:Use of a conditionally lethal gene in Anabaena sp. strain PCC 7120 to select for double recombinants and to entrap insertion sequences. Cai YP, Wolk CP. J Bacteriol. 1990 Jun;172(6):3138-45. PubMed 2160938