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Tension-tuned receptors for synthetic mechanotransduction and intercellular force detection.
Sloas DC, Tran JC, Marzilli AM, Ngo JT
Nat Biotechnol. 2023 Jan 16. doi: 10.1038/s41587-022-01638-y. PubMed Article

Plasmids from Article

ID Plasmid Purpose
190801LV UAS-DsRed-Express2-PGK-PuroLV construct encoding UAS-DsRed-Express2 reporter gene.
194929LV anti-FITC-LaG16-NRR-T2A-BFPLV construct encoding NRR-based SynNotch receptor
194930LV anti-FITC-LaG16-sNRR-T2A-BFPLV construct encoding sNRR-based SynNotch receptor
194931LV anti-FITC-LaG16-Y186F-T2A-BFPLV construct encoding Y186F mutant sNRR-based SynNotch receptor
194932LV anti-FITC-LaG16-Y186A-T2A-BFPLV construct encoding Y186A mutant sNRR-based SynNotch receptor
194933LV anti-FITC-LaG16-Y192A-T2A-BFPLV construct encoding Y192A mutant sNRR-based SynNotch receptor
194934LV anti-FITC-LaG16-Y186F/Y229A-2Xmutant-T2A-BFPLV construct encoding Y186F/Y229A double mutant sNRR-based SynNotch receptor
194935LV anti-FITC-LaG16-R103A-T2A-BFPLV construct encoding R103A mutant sNRR-based SynNotch receptor
194936LV TRE-GFP-TMD-DLL1LV construct encoding GFP-TMD-DLL1 ligand under dox-inducible TRE promoter and containing a PGK-rtTA-M2-IRES-Puro regulator/resistance marker cassette.
194937LV TRE-GFP-TMD-DLL1-Nbox-mutantLV construct encoding a mutated GFP-TMD-DLL1 ligand containing an ablated Nbox site under dox-inducible TRE promoter and containing a PGK-rtTA-M2-IRES-Puro regulator/resistance marker cassette.
195854pBAD-6xHis-AviTag-ALFAtag-eGFPBacterial expression vector encoding 6xHis-AviTag-ALFAtag-eGFP. Co-expression in cells with biotin ligase (BirA) results in efficient biotinylation of the AviTag sequence.

Antibodies from Article