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Thermodynamic analysis of the GAS(right) transmembrane motif supports energetic model of dimerization.
Diaz Vazquez G, Cui Q, Senes A
Biophys J. 2023 Jan 3;122(1):143-155. doi: 10.1016/j.bpj.2022.11.018. Epub 2022 Nov 12. PubMed Article

Plasmids from Article

ID Plasmid Purpose
191238pSN-A112C-GpAExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA and a His-tag, for fluorescent studies.
191239pSN-A112C-GpA-G83IExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA-G83I and a His-tag, for fluorescent studies.
191240pSN-A112C-CP8B1Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-CP8B1 and a His-tag, for fluorescent studies.
191241pSN-A112C-1A32Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A32 and a His-tag, for fluorescent studies.
191242pSN-A112C-1A31Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A31 and a His-tag, for fluorescent studies.

Antibodies from Article