Microtubule retrograde flow retains neuronal polarization in a fluctuating state.
Schelski M, Bradke F
Sci Adv. 2022 Nov 4;8(44):eabo2336. doi: 10.1126/sciadv.abo2336. Epub 2022 Nov 4. PubMed Article
Schelski M, Bradke F
Sci Adv. 2022 Nov 4;8(44):eabo2336. doi: 10.1126/sciadv.abo2336. Epub 2022 Nov 4. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 191320 | pBetaActin-TUBB2a-mEos3.2 | Expresses fuorescently labeled beta tubulin to allow photoconversion of microtubule patches from green to red fluorescent with UV light |
| 191321 | pBetaActin-TUBB2a-Dronpa | Expresses fuorescently labeled beta tubulin to allow photoactivation of microtubule patches with very little UV light |
| 191322 | pBetaActin-mNeonGreen-CAMSAP3 | Expresses the fluorescently labeled microtubule minus end marker CAMSAP3 to measure microtubule retrograde flow |
| 191323 | pBetaActin-TUBB2a-mNeonGreen | Expresses fluorescent labeled beta Tubulin to measure microtubule density |
| 191324 | pBetaActin-TUBB2a-mScarlet | Expresses fluorescent labeled beta Tubulin to measure microtubule density |
| 191325 | pBetaActin-IC2N-dCer3 | Chronically inhibit dynein by expressing the dominant negative acting N-terminal domain of the dynein intermediate chain (IC2N) |
| 191326 | pBetaActin-FRB-dCer3-CAAX | Expresses the fluorescently labeled membrane anchor CAAX fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB |
| 191327 | pBetaActin-dCer3-C2-FRB | Expresses the fluorescently labeled membrane anchor C2 fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB |
| 191328 | pBetaActin-dCer3-C2-dCer3 | Expresses the fluorescently labeled membrane anchor C2 without the chemical dimerization domain FRB as a control for recruiting proteins to plasma membrane |
| 191329 | pBetaActin-Halo-CAMSAP3 | Expresses the fluorescently labeled microtubule minus end marker CAMSAP3 to measure microtubule retrograde flow |
| 191330 | pBetaActin-FKBP-mScarlet-Dync1h1motor | Expresses the fluorescently labeled dynein motor domain fused to dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB |
| 191331 | pBetaActin-FKBP-mScarlet-Dync1h1motorK2599T | Expresses the fluorescently labeled motor-deficient dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB |
| 191332 | pBetaActin-FKBP-Dync1h1motor | Expresses the dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB |
| 191333 | pBetaActin-FKBP-Halo-Dync1h1motor | Expresses the fluorescently labeled dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB |
| 191334 | pBetaActin-FKBP-mScarlet-KIFC1motor | Expresses the fluorescently labeled minus-end directed KIFC1 motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB |
| 191335 | pBetaActin-FKBP-mScarlet-KIFC1motorN593K | Expresses the fluorescently labeled minus-end directed KIFC1 motor-deficient motor domain fused to FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB |
| 191336 | pBetaActin-KIF1aMotor-mScarlet-FKBP | Expresses the fluorescently labeled plus-end directed KIF1a motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB |
| 191337 | pBetaActin-KIF13aMotor-mScarlet-FKBP | Expresses the fluorescently labeled plus-end directed KIF13a motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB |
| 191338 | pBetaActin-TUBB2a-mEos3.2-mEos3.2-mEos3.2 | Expresses triple fuorescently labeled beta tubulin to allow photoconversion of microtubule patches from green to red fluorescent with UV light in slice cultures |