GATA2 mitotic bookmarking is required for definitive haematopoiesis.
Silverio-Alves R, Kurochkin I, Rydstrom A, Vazquez Echegaray C, Haider J, Nicholls M, Rode C, Thelaus L, Lindgren AY, Ferreira AG, Brandao R, Larsson J, de Bruijn MFTR, Martin-Gonzalez J, Pereira CF
Nat Commun. 2023 Aug 14;14(1):4645. doi: 10.1038/s41467-023-40391-x. PubMed Article
Silverio-Alves R, Kurochkin I, Rydstrom A, Vazquez Echegaray C, Haider J, Nicholls M, Rode C, Thelaus L, Lindgren AY, Ferreira AG, Brandao R, Larsson J, de Bruijn MFTR, Martin-Gonzalez J, Pereira CF
Nat Commun. 2023 Aug 14;14(1):4645. doi: 10.1038/s41467-023-40391-x. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 193060 | pFUW-tetO-mCherry | doxycycline-inducible expression of mCherry in mammalian cell |
| 193061 | pFUW-tetO-mCh-Gata2 | doxycycline-inducible expression of mCherry fused to mouse Gata2 in mammalian cell |
| 193062 | pFUW-tetO-mCh-Gfi1b | doxycycline-inducible expression of mCherry fused to mouse Gfi1b in mammalian cells |
| 193063 | pFUW-teto-mch-cFos | doxycycline-inducible expression of mCherry fused to mouse cFos in mammalian cell |
| 193064 | pHAGE-Gata2-mCh | constitutive expression of mouse Gata2 fused to mCherry in mammalian cells |
| 193065 | pHAGE-Gfi1b-mCh | constitutive expression of mouse Gfi1b fused to mCherry in mammalian cells |
| 193066 | pHAGE-cFos-mCh | constitutive expression of mouse cFos fused to mCherry in mammalian cells |
| 193067 | pFUW-tetO-mCh-Gata2-del-N-Terminal(1-235) | doxycycline-inducible expression of mouse Gata2 without the N-terminal (amino acids 1-235), in mammalian cells |
| 193068 | pFUW-tetO-mCh-Gata2-del-N-ZF(287-342) | doxycycline-inducible expression of mouse Gata2 without the N-terminal zinc finger (amino acids 287-342), in mammalian cells |
| 193069 | pFUW-tetO-mCh-Gata2-del-C-ZF(343-379) | doxycycline-inducible expression of mouse Gata2 without the C-terminal zinc finger (amino acids 343-379), in mammalian cells |
| 193070 | pFUW-tetO-mCh-Gata2-del-NLS(380-440) | doxycycline-inducible expression of mouse Gata2 without the nuclear localisation signal (amino acids 380-440), in mammalian cells |
| 193071 | SFFV-mCh-GATA2 | constitutive expression of mCherry fused to human Gata2 in mammalian cells |
| 193072 | SFFV-mCh-GATA2-R293Q | constitutive expression of mCherry fused to human Gata2 with mutation R293Q in mammalian cells |
| 193073 | SFFV-mCh-GATA2-P304H | constitutive expression of mCherry fused to human Gata2 with mutation P304H in mammalian cells |
| 193074 | SFFV-mCh-GATA2-R307W | constitutive expression of mCherry fused to human Gata2 with mutation R307W in mammalian cells |
| 193075 | SFFV-mCh-GATA2-A318T | constitutive expression of mCherry fused to human Gata2 with mutation A318T in mammalian cells |
| 193076 | SFFV-mCh-GATA2-L321F | constitutive expression of mCherry fused to human Gata2 with mutation L321F in mammalian cells |
| 193077 | SFFV-mCh-GATA2-R330Q | constitutive expression of mCherry fused to human Gata2 with mutation R330Q in mammalian cells |
| 193078 | SFFV-mCh-GATA2-T354M | constitutive expression of mCherry fused to human Gata2 with mutation T354M in mammalian cells |
| 193079 | SFFV-mCh-GATA2-L359V | constitutive expression of mCherry fused to human Gata2 with mutation L359V in mammalian cells |
| 193080 | SFFV-mCh-GATA2-R361L | constitutive expression of mCherry fused to human Gata2 with mutation R361L in mammalian cells |
| 193081 | SFFV-mCh-GATA2-R362Q | constitutive expression of mCherry fused to human Gata2 with mutation R362Q in mammalian cells |
| 193082 | SFFV-mCh-GATA2-C373R | constitutive expression of mCherry fused to human Gata2 with mutation C373R in mammalian cells |
| 193083 | SFFV-mCh-GATA2-R396Q | constitutive expression of mCherry fused to human Gata2 with mutation R396Q in mammalian cells |
| 193084 | SFFV-mCh-GATA2-R398W | constitutive expression of mCherry fused to human Gata2 with mutation R398W in mammalian cells |
| 193085 | FUW-Gata2-C373R | constitutive expression of mouse Gata2 with mutation C373R in mammalian cells |
| 193086 | FUW-Gata2-L359V | constitutive expression of mouse Gata2 with mutation L359V in mammalian cells |
| 193087 | FUW-Gata2 | constitutive expression of mouse Gata2 in mammalian cells |
| 193088 | FUW-Gfi1b | constitutive expression of mouse Gfi1b in mammalian cells |
| 193089 | FUW-cFos | constitutive expression of mouse cFos in mammalian cells |
| 193090 | FUW-MD-Gata2 | constitutive expression of the mitosis degradation (MD) domain of cyclin B1 fused to mouse Gata2 in mammalian cells |
| 193091 | FUW-MDmut-Gata2 | constitutive expression of a mutated (mut, R42A), non-functional version of the mitosis degradation (MD) domain of cyclin B1 fused to mouse Gata2 in mammalian cells |
| 193092 | FUW-mTurquoise | constitutive expression of mTurquoise in mammalian cells |
| 193093 | FUW-H2B-mTurquoise | constitutive expression of H2B-mTurquoise in mammalian cells |
| 193094 | FUW-MD-mTurquoise | constitutive expression of the mitosis degradation (MD) domain of cyclin B1 fused to mTurquoise in mammalian cells |
| 193095 | FUW-MDmut-mTurquoise | constitutive expression of a mutated (mut, R42A), non-functional version of the mitosis degradation (MD) domain of cyclin B1 fused to mTurquoise in mammalian cells |