A conformational switch in clathrin light chain regulates lattice structure and endocytosis at the plasma membrane of mammalian cells.
Obashi K, Sochacki KA, Strub MP, Taraska JW
Nat Commun. 2023 Feb 9;14(1):732. doi: 10.1038/s41467-023-36304-7. PubMed Article
Obashi K, Sochacki KA, Strub MP, Taraska JW
Nat Commun. 2023 Feb 9;14(1):732. doi: 10.1038/s41467-023-36304-7. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 193552 | EGFP-ShadowY-CLC | Expresses mouse Clathrin Light Chain A tagged with mEGFP and ShadowY in mammalian cells. |
| 193553 | EGFP-mCherry | Expresses mEGFP-mCherry in mammalian cells. |
| 193554 | EGFP-Scarlet-I | Expresses mEGFP-mScarlet-I in mammalian cells. |
| 193555 | EGFP-ShadowY | Expresses mEGFP-ShadowY in mammalian cells. |
| 193556 | mNeonGreen-Scarlet-I | Expresses mNeonGreen-mScarlet-I in mammalian cells. |
| 193557 | mTurquoise2-ShadowY | Expresses mTurquoise2-ShadowY in mammalian cells. |
| 193558 | mTurquoise2-Scarlet-I | Expresses mTurquoise2-mScarlet-I in mammalian cells. |
| 193559 | EGFP-CLC | Expresses human Clathrin Light Chain B tagged with mEGFP in mammalian cells. |
| 193560 | ShadowY-CLC | Expresses human Clathrin Light Chain B tagged with ShadowY in mammalian cells. |
| 193561 | CLC-ShadowY | Expresses human Clathrin Light Chain B tagged with ShadowY in mammalian cells. |
| 193562 | EGFP-CLCdeltaN | Expresses human Clathrin Light Chain B truncation mutant (deleted 1-89 aa) tagged with mEGFP in mammalian cells. |
| 193563 | EGFP-QQN | Expresses human Clathrin Light Chain B mutant (residues 20-22 were substituted from EED to QQN) tagged with mEGFP in mammalian cells. |
| 193564 | EGFP-nCLC | Expresses human Clathrin Light Chain B (neuronal isoform) tagged with mEGFP in mammalian cells. |
| 193565 | nCLC-ShadowY | Expresses human Clathrin Light Chain B (neuronal isoform) tagged with ShadowY in mammalian cells. |
| 193566 | CLC-EGFP | Expresses human Clathrin Light Chain B tagged with mEGFP in mammalian cells. |
| 193567 | FKBP-EGFP-CLC | Expresses human Clathrin Light Chain B tagged with FKBP and mEGFP in mammalian cells. |
| 193568 | PH-miRFP-FRB | Expresses PH domain from PLC delta1 tagged with miRFP703 and FRB mutant (T2098L) in mammalian cells. |
| 193569 | PH-miRFP-FRBx2 | Expresses PH domain from PLC delta1 tagged with miRFP703 and two FRB mutants (T2098L) in mammalian cells. |
| 193570 | PH-mCherry-FRBx2 | Expresses PH domain from PLC delta1 tagged with mCherry and two FRB mutants (T2098L) in mammalian cells. |
| 193571 | mCherry-FRBx2 | Expresses mCherry tagged with two FRB mutants (T2098L) in mammalian cells. |
| 193572 | CLC-FKBP-EGFP | Expresses human Clathrin Light Chain B tagged with FKBP and mEGFP in mammalian cells. |
| 193573 | CLC-EGFP206(5) | Expresses human Clathrin Light Chain B tagged with mEGFP at amino acid position 206 with a rigid alpha helix of 5 amino acids in mammalian cells. |
| 193574 | CLC-EGFP206(6) | Expresses human Clathrin Light Chain B tagged with mEGFP at amino acid position 206 with a rigid alpha helix of 6 amino acids in mammalian cells. |
| 193575 | CLC-EGFP206(7) | Expresses human Clathrin Light Chain B tagged with mEGFP at amino acid position 206 with a rigid alpha helix of 7 amino acids in mammalian cells. |
| 193576 | CLC-EGFP206(flex5) | Expresses human Clathrin Light Chain B tagged with mEGFP at amino acid position 206 with a flexible linker of 5 amino acids in mammalian cells. |
| 193577 | CLC-EGFP206(flex6) | Expresses human Clathrin Light Chain B tagged with mEGFP at amino acid position 206 with a flexible linker of 6 amino acids in mammalian cells. |
| 193578 | CLC-EGFP206(flex7) | Expresses human Clathrin Light Chain B tagged with mEGFP at amino acid position 206 with a flexible linker of 7 amino acids in mammalian cells. |
| 193579 | SNAP-CLC-EGFP | Expresses human Clathrin Light Chain B tagged with SNAP-tag and mEGFP in mammalian cells. |
| 193580 | EGFP-CLC-SNAP | Expresses human Clathrin Light Chain B tagged with SNAP-tag and mEGFP in mammalian cells. |