Addgene: RNA recording in single bacterial cells using reprogrammed tracrRNAs.

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Chase Beisel
Jiao et al

RNA recording in single bacterial cells using reprogrammed tracrRNAs.
Jiao C, Reckstadt C, Konig F, Homberger C, Yu J, Vogel J, Westermann AJ, Sharma CM, Beisel CL
Nat Biotechnol. 2023 Jan 5. doi: 10.1038/s41587-022-01604-8. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
194209	Sth1Cas9n cytosine base editor	Sth1Cas9-D9A-nickase_rAPOBEC1_cytosine base editor for RNA recording
194210	CJ8421_04975 Rptr2 and CJ8421_04975 mRNA	Expresses the Campylobacter mRNA CJ8421_04975 and the mRNA-targeting reprogrammed tracrRNA 2
194211	CJ8421_04975 Rptr2 recording plasmid	CJ8421_04975 Rptr2 targeted DNA sequence
199718	tracrRNA(scr)-CJ8421_04975 mRNA	Expresses the Campylobacter mRNA CJ8421_04975 and tracrRNA with scrambled anti-repeat sequencesequence

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