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Improved Split TEV GPCR beta-arrestin-2 Recruitment Assays via Systematic Analysis of Signal Peptide and beta-arrestin Binding Motif Variants.
Wu Y, von Hauff IV, Jensen N, Rossner MJ, Wehr MC
Biosensors (Basel). 2022 Dec 29;13(1):48. doi: 10.3390/bios13010048.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
194358pcDNA3_DRD1-NTEV-TCS-GV-2xHASplit TEV assays
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194359pcDNA3_SP-DRD1-NTEV-TCS-GV-2xHASplit TEV assays
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194360pcDNA3_DRD1-V2R-NTEV-TCS-GV-2xHASplit TEV assays
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194361pcDNA3_SP-DRD1-V2R-NTEV-TCS-GV-2xHASplit TEV assays
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194362pcDNA3_DRD2-NTEV-TCS-GV-2xHASplit TEV assays
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194363pcDNA3_SP-DRD2-NTEV-TCS-GV-2xHASplit TEV assays
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194364pcDNA3_DRD2-V2R-NTEV-TCS-GV-2xHASplit TEV assays
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194365pcDNA3_SP-DRD2-V2R-NTEV-TCS-GV-2xHASplit TEV assays
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194366pcDNA3_HTR2A-NTEV-TCS-GV-2xHASplit TEV assays
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194367pcDNA3_SP-HTR2A-NTEV-TCS-GV-2xHASplit TEV assays
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194368pcDNA3_HTR2A-V2R-NTEV-TCS-GV-2xHASplit TEV assays
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194369pcDNA3_SP-HTR2A-V2R-NTEV-TCS-GV-2xHASplit TEV assays
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194370pcDNA3_AVPR2-NTEV-TCS-GV-2xHASplit TEV assays
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194371pcDNA3_SP-AVPR2-NTEV-TCS-GV-2xHASplit TEV assays
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194372pcDNA3_AVPR2-V2R-NTEV-TCS-GV-2xHASplit TEV assays
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194373pcDNA3_SP-AVPR2-V2R-NTEV-TCS-GV-2xHASplit TEV assays
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194374pcDNA3_GCGR-NTEV-TCS-GV-2xHASplit TEV assays
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194375pcDNA3_SP-GCGR-NTEV-TCS-GV-2xHASplit TEV assays
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194376pcDNA3_GCGR-V2R-NTEV-TCS-GV-2xHASplit TEV assays
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194377pcDNA3_SP-GCGR-V2R-NTEV-TCS-GV-2xHASplit TEV assays
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194378pcDNA3_GLP1R-NTEV-TCS-GV-2xHASplit TEV assays
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194379pcDNA3_SP-GLP1R-NTEV-TCS-GV-2xHASplit TEV assays
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194380pcDNA3_GLP1R-V2R-NTEV-TCS-GV-2xHASplit TEV assays
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194381pcDNA3_SP-GLP1R-V2R-NTEV-TCS-GV-2xHASplit TEV assays
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194382pcDNA3.1_Zeo_ARRB2-1-383-CTEV-2xHASplit TEV assays
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194383pGL4_10xUAS-MLPmin-luc2Reporter plasmid for split TEV assays; firefly luciferase reporter gene driven by 10x clustered UAS binding sites linked to a minimal MLP promoter
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194384pGL4_CRE-CMVmin-luc2Reporter plasmid for cAMP/Calcium assays; firefly luciferase reporter gene driven by 6x clustered CRE binding sites linked to a minimal CMV promoter
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194385pcDNA3_attR1-ORF-attR2-NTEV-TCS-GV-2xHA_DESTGateway Destination vector for split TEV assays
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194386pcDNA3_attR1-ORF-attR2-V2R-NTEV-TCS-GV-2xHA_DESTGateway Destination vector for GPCR split TEV assays with V2R tail
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Antibodies from Article