GPI-anchored ligand-BioID2-tagging system identifies Galectin-1 mediating Zika virus entry.
Gao SS, Shi R, Sun J, Tang Y, Zheng Z, Li JF, Li H, Zhang J, Leng Q, Xu J, Chen X, Zhao J, Sy MS, Feng L, Li C
iScience. 2022 Nov 2;25(12):105481. doi: 10.1016/j.isci.2022.105481. eCollection 2022 Dec 22. PubMed Article
Gao SS, Shi R, Sun J, Tang Y, Zheng Z, Li JF, Li H, Zhang J, Leng Q, Xu J, Chen X, Zhao J, Sy MS, Feng L, Li C
iScience. 2022 Nov 2;25(12):105481. doi: 10.1016/j.isci.2022.105481. eCollection 2022 Dec 22. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 194750 | pcDNA3.1-MCS-13 x Linker-BioID2-HA-GPI-PSS | To fuse your protein of interest to the N-terminus of GPI-anchored-BioID2 |
| 194751 | pcDNA3.1-MCS-PrP (1-22)-BioID2-HA-13 x Linker-GPI-PSS | To fuse your protein of interest to the C-terminus of GPI-anchored-BioID2 |
| 194752 | pHAGE-PrP (1-22)-RBP(V)-13 x Linker-BioID2-HA-GPI-PSS | Lentiviral expression of RBP(V)-13 x Linker-BioID2-HA on the cell surface via a GPI anchor |
| 194753 | pHAGE-PrP (1-22)-BioID2-HA-13 x Linker- RBP(V)-GPI-PSS | Lentiviral expression of BioID2-HA-13 x Linker- RBP(V) on the cell surface via a GPI anchor |
| 194755 | pHAGE-PrP (1-22)-BioID2-HA-13 x Linker- RBP(Z)-GPI-PSS | Lentiviral expression of BioID2-HA-13 x Linker- RBP(Z) on the cell surface via a GPI anchor |