pcDNA3.1-MCS-PrP (1-22)-BioID2-HA-13 x Linker-GPI-PSS
(Plasmid #194751)

• Purpose: (Empty Backbone) To fuse your protein of interest to the C-terminus of GPI-anchored-BioID2
• Depositing Lab: Chaoyang Li
• Publication: Gao et al iScience. 2022 Nov 2;25(12):105481. doi: 10.1016/j.isci.2022.105481. eCollection 2022 Dec 22.

Backbone

• Vector backbone: pcDNA3.1-MCS-13_Linker-BioID2-HA-GPI-PSS
• Backbone manufacturer: Chaoyang Li lab
• Backbone size (bp): 6452
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)
• Tag / Fusion Protein:
  • HA (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Tag / Fusion Protein:
  • HA (N terminal on backbone)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Addgene Plasmid #80899

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The plasmid of pcDNA3.1 MCS-13 x Linker-BioID2-HA-GPI-PSS was mutated by adding restriction enzyme recognition sequence for XbaI between in 1181 and 1182 site. Then the BioID2-HA fragment was deleted to generate pcDNA3.1-MCS-13 x Linker-GPI-PSS. Next, PrP (1-22)-BioID2-HA was amplified by overlap PCR. The fragments were digested with NheI and EcoRI and then ligated into plasmid of pcDNA3.1-MCS-13 x Linker-GPI-PSS, PrP (1-22)-BioID2-HA, 789 bp. 5' Cloning Site: NheI (not destroyed), 3' Cloning Site: EcoRI (not destroyed)