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Ras drives malignancy through stem cell crosstalk with the microenvironment.
Yuan S, Stewart KS, Yang Y, Abdusselamoglu MD, Parigi SM, Feinberg TY, Tumaneng K, Yang H, Levorse JM, Polak L, Ng D, Fuchs E
Nature. 2022 Nov 30. doi: 10.1038/s41586-022-05475-6.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
195332SBE-NLS-mCherry PGK-rtTA3Lentiviral construct of TGFβ reporter (nuclear mCherry) and hPGK driven rtTA3
195881TRE-VEGFA EF1a-rtTA3Lentiviral construct of doxycycline inducible VEGFA
195882TRE-STOP EF1a-rtTA3control construct of TRE-VEGFA EF1a-rtTA3
195883hPGK-rtTA3Lentiviral construct of hPGK driven rtTA3
196584Lepr peak reporter-eGFPCis-regulatory region of mouse Lepr gene driven a eGFP reporter with hGFP driven rtTA3
196635TRE-Lepr_FL-IRES-eGFPDoxycycline inducible expression of full length mouse Lepr with eGFP marker
196935TRE-Lepr_Short-IRES-eGFPDoxycycline inducible expression of short Lepr (no signaling) with eGFP marker

Antibodies from Article