Curing "GFP-itis" in Bacteria with Base Editors: Development of a Genome Editing Science Program Implemented with High School Biology Students.
Vasquez CA, Evanoff M, Ranzau BL, Gu S, Deters E, Komor AC
CRISPR J. 2023 Apr 20. doi: 10.1089/crispr.2023.0002. PubMed Article
Vasquez CA, Evanoff M, Ranzau BL, Gu S, Deters E, Komor AC
CRISPR J. 2023 Apr 20. doi: 10.1089/crispr.2023.0002. PubMed Article
These materials are included in the Komor - GFP-itis Bacterial Base Editor Activity Kit.
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 195342 | GFP-itis pBE-t | Base editor plasmid expressing ABE8e-NG-Cas9n under Theophylline Riboswitch and constituitively expressed gRNA targeting EGFP A110V |
| 195343 | GFP-itis pBE-nt | Base editor plasmid expressing ABE8e-NG-Cas9n under Theophylline Riboswitch and constituitively expressed nonsense non-targeting gRNA |
| 195344 | GFP-itis pSel | Selection plasmid expressing non-fluorescent EGFP A111V |