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Next-generation plasmids for transgenesis in zebrafish and beyond.
Kemmler CL, Moran HR, Murray BF, Scoresby A, Klem JR, Eckert RL, Lepovsky E, Bertho S, Nieuwenhuize S, Burger S, D'Agati G, Betz C, Puller AC, Felker A, Ditrychova K, Botschi S, Affolter M, Rohner N, Lovely CB, Kwan KM, Burger A, Mosimann C
Development. 2023 Mar 28:dev.201531. doi: 10.1242/dev.201531.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
195948pCK033 p5E desma MCSGateway p5E vector
195949pCK011 p5E exorhGateway p5E vector
195950pCK072 p5E 147bp exorh:EGFPGateway p5E vector with reporter
195951pCK074 p5E exorh:EGFPGateway p5E vector with reporter
195952pCK051 p5E exorh:mCeruleanGateway p5E vector with reporter
195953pCK052 p5E exorh:mCherry Gateway p5E vector with reporter
195956pCK002 pME mCeruleanGateway pME fluorophore
195957769 pME mCerulean-CAAXGateway pME fluorophore
195958765 pME nls mCeruleanGateway pME fluorophore
195959pSN001 pME minprom_mCeruleanGateway pME fluorophore
195960763 pME mAppleGateway pME fluorophore
195961768 pME mApple-CAAXGateway pME fluorophore
195962764 pME nls-mAppleGateway pME fluorophore
195964pME minprom KalTa4Gateway pME Gal4 variant
195965pCK007 pME KalTa4Gateway pME Gal4 variant
195966pME minprom_creERT2Gateway pME CreERT2 ORF
195967457 p3E-2A-EGFPpAGateway p3E 2A fluorophore
195968458 p3E-2A-EGFP-CAAXpAGateway p3E 2A fluorophore
195969459 p3E-2A-nlsEGFPpAGateway p3E 2A fluorophore
195970460 p3E-2A-mCherrypAGateway p3E 2A fluorophore
195971612 p3E-2A-mCherryCAAXpAGateway p3E 2A fluorophore
195972766 p3E-2A-nlsmCherrypAGateway p3E 2A fluorophore
195973791 p3E-2A-pCS2MCSpA v2Gateway p3E 2A fluorophore
195974pAF020 p3E-2A-mCeruleanGateway p3E 2A fluorophore
195977p3E ubb polyAGateway p3E vector
195978pCK004 ubi:mCerulean_SV40 (#302)Expression construct
195979pCK005 ubi:mCerulean_ubb-polyAExpression construct
195980pCK029 exorh:EGFPExpression construct
195981pCK036 desmaMCS:minCerulean,cryaa:VenusExpression construct
195982pCK069 desmaMCS:minprom_mApple,exorh:EGFPExpression construct
195983pCK017 pDEST exorh:EGFPTransgenesis backbone
195985pCK054 pDEST exorh:mCeruleanTransgenesis backbone
195986pCB59 pDEST crybb1:mKate2Transgenesis backbone
195987pCB24 pDEST crybb1:TagBFPTransgenesis backbone
195988pDEST exorh:mGreenLantern Transgenesis backbone
195989pDEST exorh:mCherryTransgenesis backbone
200014pCK081 desmaMCS:minpromCerulean,exorh:mCherryExpression construct with desmin a regulatory elements, mouse beta-globin minimal promoter mCerulean, exorh:mCherry
200016pCK083 desmaMCS:minpromEGFP,exorh:mCherryExpression construct with desmin a regulatory elements, mouse beta-globin minimal promoter EGFP, exorh:mCherry

Antibodies from Article