Next-generation plasmids for transgenesis in zebrafish and beyond.
Kemmler CL, Moran HR, Murray BF, Scoresby A, Klem JR, Eckert RL, Lepovsky E, Bertho S, Nieuwenhuize S, Burger S, D'Agati G, Betz C, Puller AC, Felker A, Ditrychova K, Botschi S, Affolter M, Rohner N, Lovely CB, Kwan KM, Burger A, Mosimann C
Development. 2023 Mar 28:dev.201531. doi: 10.1242/dev.201531. PubMed Article
Kemmler CL, Moran HR, Murray BF, Scoresby A, Klem JR, Eckert RL, Lepovsky E, Bertho S, Nieuwenhuize S, Burger S, D'Agati G, Betz C, Puller AC, Felker A, Ditrychova K, Botschi S, Affolter M, Rohner N, Lovely CB, Kwan KM, Burger A, Mosimann C
Development. 2023 Mar 28:dev.201531. doi: 10.1242/dev.201531. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 195948 | pCK033 p5E desma MCS | Gateway p5E vector |
| 195949 | pCK011 p5E exorh | Gateway p5E vector |
| 195950 | pCK072 p5E 147bp exorh:EGFP | Gateway p5E vector with reporter |
| 195951 | pCK074 p5E exorh:EGFP | Gateway p5E vector with reporter |
| 195952 | pCK051 p5E exorh:mCerulean | Gateway p5E vector with reporter |
| 195953 | pCK052 p5E exorh:mCherry | Gateway p5E vector with reporter |
| 195956 | pCK002 pME mCerulean | Gateway pME fluorophore |
| 195957 | 769 pME mCerulean-CAAX | Gateway pME fluorophore |
| 195958 | 765 pME nls mCerulean | Gateway pME fluorophore |
| 195959 | pSN001 pME minprom_mCerulean | Gateway pME fluorophore |
| 195960 | 763 pME mApple | Gateway pME fluorophore |
| 195961 | 768 pME mApple-CAAX | Gateway pME fluorophore |
| 195962 | 764 pME nls-mApple | Gateway pME fluorophore |
| 195964 | pME minprom KalTa4 | Gateway pME Gal4 variant |
| 195965 | pCK007 pME KalTa4 | Gateway pME Gal4 variant |
| 195966 | pME minprom_creERT2 | Gateway pME CreERT2 ORF |
| 195967 | 457 p3E-2A-EGFPpA | Gateway p3E 2A fluorophore |
| 195968 | 458 p3E-2A-EGFP-CAAXpA | Gateway p3E 2A fluorophore |
| 195969 | 459 p3E-2A-nlsEGFPpA | Gateway p3E 2A fluorophore |
| 195970 | 460 p3E-2A-mCherrypA | Gateway p3E 2A fluorophore |
| 195971 | 612 p3E-2A-mCherryCAAXpA | Gateway p3E 2A fluorophore |
| 195972 | 766 p3E-2A-nlsmCherrypA | Gateway p3E 2A fluorophore |
| 195973 | 791 p3E-2A-pCS2MCSpA v2 | Gateway p3E 2A fluorophore |
| 195974 | pAF020 p3E-2A-mCerulean | Gateway p3E 2A fluorophore |
| 195977 | p3E ubb polyA | Gateway p3E vector |
| 195978 | pCK004 ubi:mCerulean_SV40 (#302) | Expression construct |
| 195979 | pCK005 ubi:mCerulean_ubb-polyA | Expression construct |
| 195980 | pCK029 exorh:EGFP | Expression construct |
| 195981 | pCK036 desmaMCS:minCerulean,cryaa:Venus | Expression construct |
| 195982 | pCK069 desmaMCS:minprom_mApple,exorh:EGFP | Expression construct |
| 195983 | pCK017 pDEST exorh:EGFP | Transgenesis backbone |
| 195985 | pCK054 pDEST exorh:mCerulean | Transgenesis backbone |
| 195986 | pCB59 pDEST crybb1:mKate2 | Transgenesis backbone |
| 195987 | pCB24 pDEST crybb1:TagBFP | Transgenesis backbone |
| 195988 | pDEST exorh:mGreenLantern | Transgenesis backbone |
| 195989 | pDEST exorh:mCherry | Transgenesis backbone |
| 200014 | pCK081 desmaMCS:minpromCerulean,exorh:mCherry | Expression construct with desmin a regulatory elements, mouse beta-globin minimal promoter mCerulean, exorh:mCherry |
| 200015 | pCK082 desmaMCS:minprommCherry,exorh:mCerulean | Expression construct with desmin a regulatory elements, mouse beta-globin minimal promoter mCherry, exorh:mCerulean |
| 200016 | pCK083 desmaMCS:minpromEGFP,exorh:mCherry | Expression construct with desmin a regulatory elements, mouse beta-globin minimal promoter EGFP, exorh:mCherry |