GEARBOCS: An Adeno Associated Virus Tool for In Vivo Gene Editing in Astrocytes
Bindu DS, Savage JT, Brose N, Bradley L, Dimond K, Tan CX, Eroglu C
eLife. 2024, Reviewed Preprint. 13:RP103249; doi: https://doi.org/10.7554/eLife.103249.1
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 196488 | pgfaABC1D-mCherry-CAAX | To over express mCherry-CAAX under gfaABC1D promoter in astrocytes |
| 196489 | pGEARBOCS-v0-Gfap-KO | To KO Gfap in mouse astrocytes |
| 196490 | pGEARBOCS-v0- Gfap-TagIN-mCherry | To tag endogenous mouse GFAP with mCherry |
| 196491 | pGEARBOCS-v0-Gfap- TagIN-HA | To tag endogenous mouse GFAP with HA |
| 196492 | pGEARBOCS-v0-Gfap-GeneTRAP | To KO and genetrap mouse GFAP simultaneously |
| 196493 | pGEARBOCS-v0-Vamp2-GeneTRAP | To KO and genetrap mouse VAMP2 simultaneously |
| 196494 | pGEARBOCS-v0-Vamp2-TagIN-HA | To tag endogenous mouse VAMP2 with HA |
| 196495 | pGEARBOCS-v0 | CRISPR tool to target astrocytes |
| 218181 | GEARBOCS | CRISPR tool to target astrocytes |
| 218182 | GEARBOCS-SPARCL1 KO | To KO Hevin in astrocytes |
| 218183 | GEARBOCS-SPARCL1-C-mCherryTag | To tag Hevin with mCherry at its C-terminal |
| 218184 | GEARBOCS-SPARCL1-GeneTRAP | To KO and genetrap mouse Hevin simultaneously |
| 218185 | GEARBOCS-SPARCL1-N-HATag | To tag Hevin with HA at the N-terminal |
| 218186 | GEARBOCS-SPARCL1-N-mCherryTag | To tag Hevin with mCherry at the N-terminal |
| 218187 | GEARBOCS-Vamp2-GeneTRAP | To KO and genetrap mouse Vamp2 simultaneously |
| 218188 | GEARBOCS-Vamp2-Tag-HA | To tag endogenous mouse VAMP2 with HA |
| 218189 | pAAV-gfaABC1D-mCherry-CAAX | To over express mCherry-CAAX under gfaABC1D promoter in astrocytes |