Addgene: Ultradeep characterisation of translational sequence determinants refutes rare-codon hypothesis and unveils quadruplet base pairing of initiator tRNA and transcript.

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Markus Jeschek
Hollerer et al

Ultradeep characterisation of translational sequence determinants refutes rare-codon hypothesis and unveils quadruplet base pairing of initiator tRNA and transcript.
Hollerer S, Jeschek M
Nucleic Acids Res. 2023 Mar 21;51(5):2377-2396. doi: 10.1093/nar/gkad040. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
196656	pASPIre4	Derivative of pASPIre3. Contains SpeI restriction site within the CDS of bxb1 to enable diversification of the 5’-UTR and codons 2-16.
196657	ptRNAfMet-A37	Plasmid for overexpression of native initiator tRNA (A37)
196658	ptRNAfMet-A37G	Plasmid for overexpression of mutated initiator tRNA (A37G)
196659	ptRNAfMet-A37U	Plasmid for overexpression of mutated initiator tRNA (A37U)

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