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Single-molecule imaging reveals translation-dependent destabilization of mRNAs.
Dave P, Roth G, Griesbach E, Mateju D, Hochstoeger T, Chao JA
Mol Cell. 2023 Feb 16;83(4):589-606.e6. doi: 10.1016/j.molcel.2023.01.013. Epub 2023 Feb 1. PubMed Article

Plasmids from Article

ID Plasmid Purpose
196918SL-TREATThe TREAT reporter containing cap-proximal GC-rich stemloop
196919SL-SunTagThe SunTag-Renilla-MS2 reporter containing cap-proximal GC-rich stem-loop
196920IRE-SunTagThe 5UTR of the ferritin heavy chain mRNA was cloned upstream of the SunTag-Renilla coding sequence in the ΔIRE-SunTag reporter
196921IRE-TREATThe 5UTR of the ferritin heavy chain mRNA was cloned upstream of the Renilla coding sequence in the ΔIRE-TREAT reporter
196922IRE-nonOpt-TREATThe codon optimized Renilla coding sequence in the IRE-TREAT reporter was replaced by non-optimal Renilla containing 93 synonymous codon substitutions.
196923IRE-TPI-wildtype-TREATThe coding sequence of Triose phosphate isomerase gene was fused downstream Renilla in the ORF
196925Let7-IRE-TREATLet7-miRNA binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT reporter.
196926IRE-let7-SunTag-TREATSunTag cassette was added in the coding region in the let7-IRE-TREAT reporter
196927IRE-let7R-SunTag-TREATSunTag cassette was added in the coding region in the let7R-IRE-TREAT reporter.
196928Let7-SunTagLet7-miRNA binding sites (3x) were integrated in between the stop codon and MS2 stem-loops in the SunTag reporter.
196929Let7R-SunTagReverse complement of let7-miRNA binding sites (3x) were integrated in between the stop codon and MS2 stem-loops in the SunTag reporter.
196930miR21-IRE-TREATmir21 binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT reporter.
196931miR21mutant-IRE-TREATMutated mir21 binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT reporter
196932IRE-miR21-SunTag-TREATSunTag cassette was added in the coding region in the miR21-IRE-TREAT reporter.
196933IRE-miR21mutant-SunTag-TREATSunTag cassette was added in the coding region in the miR21mutant -IRE-TREAT reporter.
196934Let7R-IRE-TREATReverse complement of let7-miRNA binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT.

Antibodies from Article