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Selection of extended CRISPR RNAs with enhanced targeting and specificity.
Herring-Nicholas A, Dimig H, Roesing MR, Josephs EA
Commun Biol. 2024 Jan 12;7(1):86. doi: 10.1038/s42003-024-05776-8. PubMed Article

Plasmids from Article

ID Plasmid Purpose
196986pSECRETS-AFor SECRETS protocol to screen for gRNA activity and specificity: bacterial expression Cas9 in the presence of anhydrotetracycline (aTc).
196987pSECRETS-BFor SECRETS protocol to screen for gRNA activity and specificity: BsaI cassette for gRNA expression or for cloning (x-)gRNA librarys + off-target for SECRETS counter-selection.

Antibodies from Article