Incorporation of a Doubly Functionalized Synthetic Amino Acid into Proteins for Creating Chemical and Light-Induced Conjugates.
Yamaguchi A, Matsuda T, Ohtake K, Yanagisawa T, Yokoyama S, Fujiwara Y, Watanabe T, Hohsaka T, Sakamoto K
Bioconjug Chem. 2016 Jan 20;27(1):198-206. doi: 10.1021/acs.bioconjchem.5b00602. Epub 2015 Dec 16. PubMed Article
Yamaguchi A, Matsuda T, Ohtake K, Yanagisawa T, Yokoyama S, Fujiwara Y, Watanabe T, Hohsaka T, Sakamoto K
Bioconjug Chem. 2016 Jan 20;27(1):198-206. doi: 10.1021/acs.bioconjchem.5b00602. Epub 2015 Dec 16. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 197100 | pCDF-Mm2 | To express the variant of Methanosarcina mazei PylRS specific for pyrrolysine derivatives (Boc-Lys, Az-ZLys, AzAmZLys) and its cognate Pyl tRNA and allow UAG codon to be translated into these derivati |
| 197101 | pCDF-Az | To express the azidophenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into azidophenylalanine |