pCDF-Az
(Plasmid
#197101)
-
PurposeTo express the azidophenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into azidophenylalanine
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 197101 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepCDF-1b
-
Backbone manufacturerNovagen/Sigma Aldrich
- Total vector size (bp) 3000
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameTyrRS variant, amber suppressor tRNA, kanamycin resistance gene
-
SpeciesMethanocaldococcus jannaschii (TyrRS, tRNA), Bacteriai (Kanamycin)
-
MutationThr32, Asn107, Pro158, Leu159, Gln162, Arg286 (TyrRS)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCDF-Az was a gift from Kensaku Sakamoto (Addgene plasmid # 197101 ; http://n2t.net/addgene:197101 ; RRID:Addgene_197101) -
For your References section:
Incorporation of a Doubly Functionalized Synthetic Amino Acid into Proteins for Creating Chemical and Light-Induced Conjugates. Yamaguchi A, Matsuda T, Ohtake K, Yanagisawa T, Yokoyama S, Fujiwara Y, Watanabe T, Hohsaka T, Sakamoto K. Bioconjug Chem. 2016 Jan 20;27(1):198-206. doi: 10.1021/acs.bioconjchem.5b00602. Epub 2015 Dec 16. 10.1021/acs.bioconjchem.5b00602 PubMed 26625213
