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(Plasmid #197099)


Item Catalog # Description Quantity Price (USD)
Plasmid 197099 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    TaKaRa Bio Inc.
  • Total vector size (bp) 3100
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
    TyrRS variant, Nap3 tRNA
  • Species
    Methanocaldococcus jannaschii (TyrRS, tRNA)
  • Mutation
    Ala70, Thr158, Ser159, Arg286 (TyrRS)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To express the variant of Methanocaldococcus jannaschii TyrRS specific for halogenated tyrosines (3-iodotyrosine, 3-bromotyrosine, and 3-chlorotyrosine) and Nap3 amber suppressor tRNA and allow UAG codon to be translated into these derivatives.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIYN3 was a gift from Kensaku Sakamoto (Addgene plasmid # 197099 ; ; RRID:Addgene_197099)
  • For your References section:

    Genetic-code evolution for protein synthesis with non-natural amino acids. Mukai T, Yanagisawa T, Ohtake K, Wakamori M, Adachi J, Hino N, Sato A, Kobayashi T, Hayashi A, Shirouzu M, Umehara T, Yokoyama S, Sakamoto K. Biochem Biophys Res Commun. 2011 Aug 12;411(4):757-61. doi: 10.1016/j.bbrc.2011.07.020. Epub 2011 Jul 18. 10.1016/j.bbrc.2011.07.020 PubMed 21782790