pIYN3
(Plasmid #197099)

• Purpose: To express the variant of M. jannaschii TyrRS specific for halogenated tyrosines and Nap3 amber suppressor tRNA and allow UAG codon to be translated into these derivatives.
• Depositing Lab: Kensaku Sakamoto
• Publication: Mukai et al Biochem Biophys Res Commun. 2011 Aug 12;411(4):757-61. doi: 10.1016/j.bbrc.2011.07.020. Epub 2011 Jul 18.

Backbone

• Vector backbone: pBR322
• Backbone manufacturer: TaKaRa Bio Inc.
• Total vector size (bp): 3100
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: TyrRS variant, Nap3 tRNA
• Species: Methanocaldococcus jannaschii (TyrRS, tRNA)
• Mutation: Ala70, Thr158, Ser159, Arg286 (TyrRS)

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

To express the variant of Methanocaldococcus jannaschii TyrRS specific for halogenated tyrosines (3-iodotyrosine, 3-bromotyrosine, and 3-chlorotyrosine) and Nap3 amber suppressor tRNA and allow UAG codon to be translated into these derivatives.