Tuning phenylalanine fluorination to assess aromatic contributions to protein function and stability in cells.
Galles GD, Infield DT, Clark CJ, Hemshorn ML, Manikandan S, Fazan F, Rasouli A, Tajkhorshid E, Galpin JD, Cooley RB, Mehl RA, Ahern CA
Nat Commun. 2023 Jan 4;14(1):59. doi: 10.1038/s41467-022-35761-w. PubMed Article
Galles GD, Infield DT, Clark CJ, Hemshorn ML, Manikandan S, Fazan F, Rasouli A, Tajkhorshid E, Galpin JD, Cooley RB, Mehl RA, Ahern CA
Nat Commun. 2023 Jan 4;14(1):59. doi: 10.1038/s41467-022-35761-w. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 197576 | pAcBac1-Mb-Fluoro-Phe B5 | Expression of Methanosarcina barkeri (Mb) Fluoro-Phe B5 tRNA synthetase/Pyl-tRNA pair for the encoding of fluorinated phenylalanine derivatives at TAG codons in HEK293 cells |
| 197577 | pAcBac1-Mb-Fluoro-Phe D6 | Expression of Methanosarcina barkeri (Mb) Fluoro-Phe D6 tRNA synthetase/Pyl-tRNA pair for the encoding of fluorinated phenylalanine derivatives at TAG codons in HEK293 cells |