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Structures of BIRC6-client complexes provide a mechanism of SMAC-mediated release of caspases.
Hunkeler M, Jin CY, Fischer ES
Science. 2023 Mar 17;379(6637):1105-1111. doi: 10.1126/science.ade5750. Epub 2023 Feb 9. PubMed Article

Plasmids from Article

ID Plasmid Purpose
197967pDARMO_CMVT_3xFLAG_BIRC6Mammalian cell expression of wt 3xFLAG-BIRC6
197968pDARMO_CMVT_3xFLAG_BIRC6_BIRmutMammalian cell expression of 3xFLAG-BIRC6_BIRmut
197969pDARMO_CMVT_3xFLAG_BIRC6_delta(Helix)Mammalian cell expression of 3xFLAG-BIRC6_delta(Helix)
197970pDARMO_CMVT_3xFLAG_BIRC6_delta(Helix)_BIRmutMammalian cell expression of 3xFLAG-BIRC6_delta(Helix)/BIRmut
197971pDARMO_CMVT_N_FLAG_UBA6Mammalian cell expression of FLAG-UBA6
197972pDARMO_CMVT_N_Strep_BIRC6Mammalian cell expression of wt Strep-BIRC6
197973pDEST_CMV_N_Flag_BIRC6Mammalian cell expression of wt FLAG-BIRC6
197974pDONR223_BIRC6Entry clone for wt BIRC6
197975pET-20b_HtrA2(delta(133))_S306A_6HisE. coli expression of HtrA2(delta133)-6His
197976pNIC-Bio2_SMAC(delta(55))_9HisE. coli expression of SMAC(delta(55))-9His
197977pNIC-Bio2_SMAC(delta(55))_Cys_9HisE. coli expression of SMAC(delta(55))_Cys-9His
197978pNIC-Bio2_MVPI_SMAC(delta(55))_9HisE. coli expression of MVPI-SMAC(delta(55))_9His

Antibodies from Article