pDARMO_CMVT_3xFLAG_BIRC6_BIRmut
(Plasmid
#197968)
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PurposeMammalian cell expression of 3xFLAG-BIRC6_BIRmut
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 197968 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepDARMO_CMVT_3xFLAG
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBIRC6
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Alt nameBRUCE
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Alt nameAPOLLON
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SpeciesH. sapiens (human)
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Insert Size (bp)14571
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MutationC328S, C331S, L1332V
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Entrez GeneBIRC6 (a.k.a. APOLLON, BRUCE)
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Tag
/ Fusion Protein
- 3xFLAG (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Electroporation recommended.
Please visit https://www.biorxiv.org/content/10.1101/2022.08.19.504532v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDARMO_CMVT_3xFLAG_BIRC6_BIRmut was a gift from Eric Fischer (Addgene plasmid # 197968 ; http://n2t.net/addgene:197968 ; RRID:Addgene_197968) -
For your References section:
Structures of BIRC6-client complexes provide a mechanism of SMAC-mediated release of caspases. Hunkeler M, Jin CY, Fischer ES. Science. 2023 Mar 17;379(6637):1105-1111. doi: 10.1126/science.ade5750. Epub 2023 Feb 9. 10.1126/science.ade5750 PubMed 36758104
