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Lysosomal GPCR-like protein LYCHOS signals cholesterol sufficiency to mTORC1.
Shin HR, Citron YR, Wang L, Tribouillard L, Goul CS, Stipp R, Sugasawa Y, Jain A, Samson N, Lim CY, Davis OB, Castaneda-Carpio D, Qian M, Nomura DK, Perera RM, Park E, Covey DF, Laplante M, Evers AS, Zoncu R
Science. 2022 Sep 16;377(6612):1290-1298. doi: 10.1126/science.abg6621. Epub 2022 Aug 25. PubMed Article

Plasmids from Article

ID Plasmid Purpose
199633pLVX LYCHOS (GPR155) WT-FLAGLentiviral construct to stably express LYCHOS (GPR155) WT
199660pRK5-LYCHOS (GPR155) WT-HATransient expression of LYCHOS (GPR155) WT
199661pRK5-LYCHOS (GPR155) FP>IA-HATransient expression of LYCHOS (GPR155) FP>IA mutant
199662pRK5-LYCHOS (GPR155) E48Q-HATransient expression of LYCHOS (GPR155) E48Q mutant
199674pRK5-LYCHOS (GPR155) F43I-HATransient expression of LYCHOS (GPR155) F43I mutant
199675pRK5-LYCHOS (GPR155) P44A-HATransient expression of LYCHOS (GPR155) P44A mutant
199676pRK5 LYCHOS (GPR155) Y551A-HATransient expression of LYCHOS (GPR155) Y551A mutant
199677pRK5-LYCHOS (GPR155) 4CA-HATransient expression of LYCHOS (GPR155) C595.604.629.638A mutant
199678pET28a LZ control-FLAGBacterial expression of Leucine Zipper (LZ) control-Flag
199679pET28a LZ LED WT-FLAGBacterial expression of Leucine Zipper (LZ) LYCHOS LED-Flag
199680pET28a LZ LED Y551A-FLAGBacterial expression of Leucine Zipper (LZ) LYCHOS LED Y551A-Flag
199681pET28a LZ LED 4CA-FLAGBacterial expression of Leucine Zipper (LZ) LYCHOS LED 4CA-Flag
199682pRK5 LYCHOS ∆LED-HATransient expression of LYCHOS (GPR155) ∆LED-HA
199683pRK5-LYCHOS (GPR155) ∆DEP-HATransient expression of LYCHOS (GPR155) ∆DEP-HA
199684pRK5-LYCHOS (GPR155) WT-mycTransient expression of LYCHOS (GPR155) WT
199685pRK5-myc Metap2Transient expression of Metap2
199686pRK5 LYCHOS (GPR155) ∆Permease-HATransient expression of LYCHOS (GPR155) ∆Permease
199687pFB-CG-LYCHOS WT-HA-GFPExpression of LYCHOS WT in insect cells
199688pFB-CG-LYCHOS FP>IA-HA-GFPExpression of LYCHOS FP>IA mutant in insect cells
199747pLVX LYCHOS-FLAG-TiDLentiviral construct to stably express LYCHOS (GPR155) TurboID
199748pLVX NPC1-FLAG-TiDLentiviral construct to stably express NPC1 TurboID
199749pLVX FLAG-SLC38A9.1Lentiviral construct to stably express SLC38A91.1
199750pLJM1-Lamp1 FLAGLentiviral construct to stably express Lamp1
209314pLKO shLYCHOS/FLAG Metap2Lentiviral construct expressing FLAG Metap2 and shRNA against LYCHOS
209315pLKO shLYCHOS/LYCHOS WT-FLAGLentiviral construct expressing shRNA against LYCHOS and LYCHOS WT-FLAG (resistant to shRNA)
209316pLKO shLuc/FLAG Metap2Lentiviral construct expressing FLAG Metap2 and shRNA against Luciferase (control shRNA)

Antibodies from Article