Specific Modulation of CRISPR Transcriptional Activators through RNA-Sensing Guide RNAs in Mammalian Cells and Zebrafish Embryos
Pelea O, Mayes S, Ferry QRV, Fulga TA, Sauka-Spengler T
bioRxiv 2023.05.08.539738 Article
Pelea O, Mayes S, Ferry QRV, Fulga TA, Sauka-Spengler T
bioRxiv 2023.05.08.539738 Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 200234 | pcDNA3.1_SV40-iBlue-pA | Backbone for cloning iSBH-sgRNAs |
| 200235 | U6-TM2Emp-6T_iBlue | Backbone for cloning short RNA triggers that activate iSBH-sgRNAs |
| 200236 | U6-TM2Emp-6T_iBlue-ModFlanks | Backbone for cloning longer RNA triggers that activate iSBH-sgRNAs |
| 200237 | sgRNA1 _1xCTS-ECFP-pA | Mammalian transfections; 1xCTS-ECFP reporter construct 1 |
| 200238 | sgRNA2 _1xCTS-ECFP-pA | Mammalian transfections; 1xCTS-ECFP reporter construct 2 |
| 200239 | sgRNA3 _1xCTS-ECFP-pA | Mammalian transfections; 1xCTS-ECFP reporter construct 3 |
| 200240 | sgRNA4 _1xCTS-ECFP-pA | Mammalian transfections; 1xCTS-ECFP reporter construct 4 |
| 200241 | sgRNA5 _1xCTS-ECFP-pA | Mammalian transfections; 1xCTS-ECFP reporter construct 5 |
| 200242 | Landing_Pad_8xCTS-ECFP-pA | Backbone for cloning 8xCTSs (8xCRISPR-target sites) |
| 200243 | sgRNA1 _8xCTS-ECFP-pA | Mammalian transfections; 8xCTS-ECFP reporter construct 1 |
| 200244 | sgRNA2_8xCTS-ECFP-pA | Mammalian transfections; 8xCTS-ECFP reporter construct 2 |
| 200245 | sgRNA3_8xCTS-ECFP-pA | Mammalian transfections; 8xCTS-ECFP reporter construct 3 |
| 200246 | gRNA4 _8xCTS-ECFP-pA | Mammalian transfections; 8xCTS-ECFP reporter construct 4 |
| 200247 | sgRNA5_8xCTS-ECFP-pA | Mammalian transfections; 8xCTS-ECFP reporter construct 5 |
| 200248 | Tol2_B-act_dCas9-Vp64-T2A-Citrine | Zebrafish transgenics; Plasmid encodes dCas9-Vp64 and could be stably integrated in the zebrafish genome using Tol2 transgenesis |
| 200249 | Tol2_8xCTS-ECFP | Zebrafish transgenics; Plasmid encodes the sgRNA2_8xCTS-ECFP reporter and could be stably integrated in the zebrafish genome using Tol2 transgenesis |