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Uncovering receptor-ligand interactions using a high-avidity CRISPR activation screening platform.
Yang L, Sheets TP, Feng Y, Yu G, Bajgain P, Hsu KS, So D, Seaman S, Lee J, Lin L, Evans CN, Guest MR, Chari R, St Croix B
Sci Adv. 2024 Feb 16;10(7):eadj2445. doi: 10.1126/sciadv.adj2445. Epub 2024 Feb 14. PubMed Article

Plasmids from Article

ID Plasmid Purpose
200832pGMC00028 (aka pTS0057)AAVS1 guide RNA cloned into the lentiSAM puro backbone
200834pGMC00029 (aka pRC0610)Lentiviral guide RNA expression plasmid for CRISPR activation with puromycin/BFP
207471FNLCR CRISPRa Cell SurfaceCRISPR-based activation library with guide RNAs targeting all cell surface proteins.
209673pGMC00030 (aka pRC0707)Lentiviral vector expressing PP7-P65-HSF1

Antibodies from Article