Addgene: A robust dual gene ON-OFF toggle directed by two independent promoter-degron pairs.

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Randy Poon
Yeung et al

A robust dual gene ON-OFF toggle directed by two independent promoter-degron pairs.
Yeung TK, Kim S, Ma HT, Poon RYC
J Cell Sci. 2023 Apr 15;136(8):jcs260754. doi: 10.1242/jcs.260754. Epub 2023 Apr 19. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
201556	pDBEcR/Bla	Plasmid with SB ITRs for expressing DBEcR (3xmyc-tagged) under EF1α promoter; blasticidin resistance.
201557	EGFP in pDBEcR-pIND(SP1)/Bla	Ecdysone-inducible vector with SB ITRs for expressing DBEcR (3xmyc- tagged) and FLAG- EGFP; blasticidin resistance.
201558	EGFP in pDBEcR-pIND(SP1)-W/Bla	Ecdysone-inducible vector with SB ITRs for expressing FLAG- EGFP (WPRE in 3' UTR) and DBEcR (3xmyc-tagged); blasticidin resistance.
201559	EGFP in pDBEcR-pIND(SP1)-DD/Bla	Ecdysone-inducible vector with SB ITRs for expressing FLAG- EGFP-DD and DBEcR (3xmyc-tagged); blasticidin resistance.
201560	EGFP in pDBEcR-pIND(SP1)-DD-W/Bla	Ecdysone-inducible vector with SB ITRs for expressing FLAG- EGFP-DD (WPRE in 3' UTR) and DBEcR (3xmyc-tagged); blasticidin resistance.

Antibodies from Article