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Diacylglycerol kinase zeta interacts with sphingomyelin synthase 1 and sphingomyelin synthase-related protein via different regions.
Furuta M, Murakami C, Numagami Y, Suzuki R, Sakane F
FEBS Open Bio. 2023 May 11. doi: 10.1002/2211-5463.13628. PubMed Article

Plasmids from Article

ID Plasmid Purpose
202526pCAGGS-N-TEV-Twin-StrepExpress C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cell
202527pCAGGS-C-TEV-Twin-StrepExpress N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cell
202528pCAGGS-SMS1-TEV-Twin-StrepExpress C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 1 (SMS1) in mammalian cell
202529pCAGGS-SMS2-TEV-Twin-StrepExpress C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 2 (SMS2) in mammalian cell
202530pCAGGS-SMSr-TEV-Twin-StrepExpress C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase-relate protein (SMSr) in mammalian cell
202531pCAGGS-Twin-Strep-TEV-DGKζExpress N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human diacylglycerol kinase ζ (DGKζ) in mammalian cell
202557pCAGGS-SMS1-TEV-HATExpress C-terminal Tobacco Etch Virus (TEV) protease cleavable HAT-tagged human Sphingomyelin synthase 1 (SMS1) in mammalian cell
202597pCAGGS-C-TEV-HATExpress N-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cell
202598pCAGGS-N-TEV-HATExpress C-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cell
203323pOET3-N-TEV-Twin-StrepGenerate the baculovirus to express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in insect cells
203324pOET3-C-TEV-Twin-StrepGenerate the baculovirus to express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in insect cells
203325pCAGGS-AcGFP-CExpress N-terminal AcGFP-fused protein in mammalian cell
203326pCAGGS-mCherry-CExpress N-terminal mCherry-fused protein in mammalian cell

Antibodies from Article