Human retinal ganglion cell neurons generated by synchronous BMP inhibition and transcription factor mediated reprogramming.
Agarwal D, Dash N, Mazo KW, Chopra M, Avila MP, Patel A, Wong RM, Jia C, Do H, Cheng J, Chiang C, Jurlina SL, Roshan M, Perry MW, Rho JM, Broyer R, Lee CD, Weinreb RN, Gavrilovici C, Oesch NW, Welsbie DS, Wahlin KJ
NPJ Regen Med. 2023 Sep 29;8(1):55. doi: 10.1038/s41536-023-00327-x. PubMed Article
Agarwal D, Dash N, Mazo KW, Chopra M, Avila MP, Patel A, Wong RM, Jia C, Do H, Cheng J, Chiang C, Jurlina SL, Roshan M, Perry MW, Rho JM, Broyer R, Lee CD, Weinreb RN, Gavrilovici C, Oesch NW, Welsbie DS, Wahlin KJ
NPJ Regen Med. 2023 Sep 29;8(1):55. doi: 10.1038/s41536-023-00327-x. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 202760 | P789_pY026_EnAsCpf1_CLYBL_T1_catRNA | Encodes for CRISPR-Cas12a (EnAsCpf1) with gRNA for targeting the CLYBL safe harbor site |
| 202761 | P931_CLYBL-CBX3-Cbh-Zeo-TetO-NAIP2 | Encodes four RGC specific transcription factors under the control of a TetOn promoter at the CLYBL safe harbor |
| 202762 | P932_CLYBL-CBX3-Cbh-Zeo-TetO-NEUROG2 | Encodes one forebrain neuron specific transcription factor under the control of a TetOn promoter at the CLYBL safe harbor |