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Addgene
Genome editing with natural and engineered CjCas9 orthologs.
Gao S, Wang Y, Qi T, Wei J, Hu Z, Liu J, Sun S, Liu H, Wang Y
Mol Ther. 2023 Apr 5;31(4):1177-1187. doi: 10.1016/j.ymthe.2023.01.029. Epub 2023 Feb 1. PubMed Article

Plasmids from Article

ID Plasmid Purpose
192125pAAV-CMV-Hsp1Cas9Expresses Hsp1Cas9
192126pAAV-CMV-Hsp2Cas9Expresses Hsp2Cas9
192127pAAV-CMV-CcuCas9Expresses CcuCas9
192128pSK-mU6-Cj-sgRNA-SV40-puroExpresses Cj-SgRNA scaffold cloned with BbsI and puromycin resistance gene
192129pSK-mU6-Hsp1-sgRNA-SV40-puroExpresses Hsp1-SgRNA scaffold cloned with BbsI and puromycin resistance gene
192130pSK-mU6-Ccu-sgRNA-SV40-puro Expresses Ccu-SgRNA scaffold cloned with BbsI and puromycin resistance gene
192131pAAV-CMV-Hsp1-Hsp2Cas9-P2A-puroExpresses Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene
192132pAAV-CMV-Hsp1-Hsp2Cas9-Y-P2A-puroExpresses highly specific Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene
192133pAAV-CMV-Hsp1-Hsp2Cas9-KY-P2A-puroExpresses highly specific Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

Antibodies from Article