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Versatile toolkit for highly-efficient and scarless overexpression of circular RNAs.
Stringer BW, Gabryelska M, Marri S, Clark L, Lin H, Gantley L, Liu R, Wilusz JE, Conn VM, Conn SJ
bioRxiv. 2023 Nov 22:2023.11.21.568171. doi: 10.1101/2023.11.21.568171. Preprint. PubMed Article

Plasmids from Article

ID Plasmid Purpose
206214mc2 neoFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).
206215mc2 puroFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).
206216mc2 hygroFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).
206217mc2 blastFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).
206218mc2 mNeonFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).
206219mc2 mCherryFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).
206220mc2 neo EF1aFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).
206221mc2 puro EF1aFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).
206222mc2 hygro EF1aFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).
206223mc2 blast EF1aFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).
206224mc2 mNeon EF1aFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).
206225mc2 mCherry EF1aFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).
206226mc2 neo hPGKFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).
206227mc2 puro hPGKFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).
206228mc2 hygro hPGKFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).
206229mc2 blast hPGKFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).
206230mc2 mNeon hPGKFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).
206231mc2 mCherry hPGKFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).
206232pF CAG mc2 IRES neoFor expressing cloned exons as circular RNA.
206233pf CAG mc2 IRES puroFor expressing cloned exons as circular RNA.
206234pF CAG mc2 IRES hygroFor expressing cloned exons as circular RNA.
206235pf CAG mc2 IRES blastFor expressing cloned exons as circular RNA.
206236pF hPGK mc2 IRES neoFor expressing cloned exons as circular RNA.
206237pf hPGK mc2 IRES puroFor expressing cloned exons as circular RNA.
206238pF hPGK mc2 IRES hygroFor expressing cloned exons as circular RNA.
206239pF hPGK mc2 IRES blastFor expressing cloned exons as circular RNA.

Antibodies from Article