pf hPGK mc2 IRES puro
(Plasmid #206237)

• Purpose: (Empty Backbone) For expressing cloned exons as circular RNA.
• Depositing Labs: Simon Conn, Brett Stringer
• Publication: Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171. doi: 10.1101/2023.11.21.568171. Preprint.

Backbone

• Vector backbone: pF CAG IRES puro
• Vector type: Mammalian Expression, Lentiviral, Synthetic Biology
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None

Resource Information

• Supplemental Documents:
  • pF hPGK mc2 IRES puro.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

ZKSCAN1 intron 1 (partial) & ZKSCAN1 intron 3 (partial) separated by a pair of BsmBI restriction sites. 5' Cloning Site: BamHI (not destroyed), 3' Cloning Site: XbaI (not destroyed). The mc2 and pF mc2 series of plasmids were designed and created by Dr Brett Stringer in the laboratory of Prof Simon Conn. They are based on the original circRNA mini vector design of Liang & Wilusz, 2014. The pair of BsmBI restriction sites is the cloning site for DNA specifying the RNA sequence to be circularised. This plasmid was created by replacing the CAG enhancer/promoter of pF CAG mc2 IRES puro with the hPGK promoter from Addgene plasmid ID 96923. Please visit https://doi.org/10.1101/2023.11.21.568171 for bioRxiv preprint.