Spatiotemporal functional assembly of split protein pairs through a light-activated SpyLigation.
Ruskowitz ER, Munoz-Robles BG, Strange AC, Butcher CH, Kurniawan S, Filteau JR, DeForest CA
Nat Chem. 2023 May;15(5):694-704. doi: 10.1038/s41557-023-01152-x. Epub 2023 Apr 17. PubMed Article
Ruskowitz ER, Munoz-Robles BG, Strange AC, Butcher CH, Kurniawan S, Filteau JR, DeForest CA
Nat Chem. 2023 May;15(5):694-704. doi: 10.1038/s41557-023-01152-x. Epub 2023 Apr 17. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 207629 | pSC-LPETG | A plasmid encoding photocaged SpyCatcher (pSC) with a C-terminal Sortase recognition sequence. |
| 207630 | nUnaG-ST | A plasmid encoding the N-terminal UnaG fragment fused to SpyTag. |
| 207631 | pSC-cUnaG | A plasmid encoding photocaged SpyCatcher (pSC) fused to C-terminal UnaG fragment. |
| 207632 | pSC-SmBiT | A plasmid encoding photocaged SpyCatcher (pSC) fused to SmBiT |
| 207633 | LgBiT-ST | A plasmid encoding LgBiT fused to SpyTag |
| 207634 | pSC-cUnaG-P2A-nUnaG-ST-P2A-mCh | A plasmid encoding photocaged SpyCatcher (pSC)-cUnaG, nUnaG-SpyTag (ST), and mCherry separated by P2A cleaving sequences for mammalian expression. |
| 207635 | pSC-cUnaG-LPETG | A plasmid encoding photocaged SpyCatcher (pSC) fused to C-terminal UnaG fragment and LPETG sortase recognition sequence. |
| 207636 | pSC-mCh-P2A-ST-EGFP-CAAX | A plasmid encoding photocaged SpyCatcher (pSC) fused to mCherry and a SpyTag-EGFP localized to the cell membrane via CAAX tag |
| 207637 | EGFP-ST-P2A-pSC-CAAX | A plasmid encoding EGFP fused to SpyTag (ST) and photocaged SpyCatcher (pSC) localized to the cell membrane via CAAX tag. |
| 207638 | nCre-ST-NLS-P2A-NLS-pSC-cCre | A plasmid encoding N-terminal Cre fused to SpyTag (ST) and photocaged SpyCatcher (pSC) fused to C-terminal Cre with a nuclear localization signal (NLS) sequence |
| 207639 | pEVOL-NBK-1 | A plasmid for the expression of M. mazei pyrrolysine aminoacyl-tRNA synthetase/tRNA pair (PylRS/tRNAPyl) in E. Coli. |