An AsCas12f-based compact genome-editing tool derived by deep mutational scanning and structural analysis.
Hino T, Omura SN, Nakagawa R, Togashi T, Takeda SN, Hiramoto T, Tasaka S, Hirano H, Tokuyama T, Uosaki H, Ishiguro S, Kagieva M, Yamano H, Ozaki Y, Motooka D, Mori H, Kirita Y, Kise Y, Itoh Y, Matoba S, Aburatani H, Yachie N, Karvelis T, Siksnys V, Ohmori T, Hoshino A, Nureki O
Cell. 2023 Sep 22:S0092-8674(23)00963-7. doi: 10.1016/j.cell.2023.08.031. PubMed Article
Hino T, Omura SN, Nakagawa R, Togashi T, Takeda SN, Hiramoto T, Tasaka S, Hirano H, Tokuyama T, Uosaki H, Ishiguro S, Kagieva M, Yamano H, Ozaki Y, Motooka D, Mori H, Kirita Y, Kise Y, Itoh Y, Matoba S, Aburatani H, Yachie N, Karvelis T, Siksnys V, Ohmori T, Hoshino A, Nureki O
Cell. 2023 Sep 22:S0092-8674(23)00963-7. doi: 10.1016/j.cell.2023.08.031. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 208356 | pCMV enAsCas12f-YHAM-sgRNA_v7 | Expression of enAsCas12f-YHAM and sgRNA_v7 in mammalian cells |
| 208357 | pCMV enAsCas12f-HKRA-sgRNA_v7 | Expression of enAsCas12f-HKRA and sgRNA_v7 in mammalian cells |