Alternative splicing of its 5' UTR limits CD20 mRNA translation and enables resistance to CD20-directed immunotherapies.
Ang Z, Paruzzo L, Hayer KE, Schmidt C, Torres-Diz M, Xu F, Zankharia U, Zhang Y, Soldan SS, Zheng S, Falkenstein CD, Loftus JP, Yang SY, Asnani M, King Sainos P, Pillai V, Chong ER, Li M, Tasian SK, Barash Y, Lieberman PM, Ruella M, Schuster SJ, Thomas-Tikhonenko A
Blood. 2023 Sep 8:blood.2023020400. doi: 10.1182/blood.2023020400. PubMed Article
Ang Z, Paruzzo L, Hayer KE, Schmidt C, Torres-Diz M, Xu F, Zankharia U, Zhang Y, Soldan SS, Zheng S, Falkenstein CD, Loftus JP, Yang SY, Asnani M, King Sainos P, Pillai V, Chong ER, Li M, Tasian SK, Barash Y, Lieberman PM, Ruella M, Schuster SJ, Thomas-Tikhonenko A
Blood. 2023 Sep 8:blood.2023020400. doi: 10.1182/blood.2023020400. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 209749 | LentiCRISPRv2-sgCD20 | Lentiviral transfer plasmid to express Cas9 and a gRNA targeting the human CD20 gene, MS4A1. |
| 209750 | LentiCRISPRv2-sgCTRL | Lentiviral transfer plasmid to express Cas9 and a control, non-specific gRNA (does not target any human gene) |
| 209751 | pCDH-EF1-IRES-BSD | Lentiviral transfer plasmid to express the blasticidin resistance gene |
| 209752 | pCDH-V1-rCD20-BSD | To reconstitute CD20 expression with a specific mRNA isoform (variant 1) after CRISPR-Cas9-mediated knockout of CD20. |
| 209753 | pCDH-V2-rCD20-BSD | To reconstitute CD20 expression with a specific mRNA isoform (variant 2) after CRISPR-Cas9-mediated knockout of CD20. |
| 209754 | pCDH-V3-rCD20-BSD | To reconstitute CD20 expression with a specific mRNA isoform (variant 3) after CRISPR-Cas9-mediated knockout of CD20. |
| 209755 | pCDH-V1-CD20-Puro | Lentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1. Specifically, the variant 1 mRNA isoform. |
| 209756 | pCDH-V2-CD20-Puro | Lentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1. Specifically, the variant 2 mRNA isoform. |
| 209757 | pCDH-V3-CD20-Puro | Lentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1. Specifically, the variant 3 mRNA isoform. |
| 209758 | pCDH-V1AUC-DelStem-CD20-Puro | Lentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1; variant 1 mRNA isoform with mutations to disrupt the uORFs and the stem-loop within the 5'-UTR. |
| 209759 | pCDH-kz-CD20-Puro | Lentiviral transfer plasmid to express the CDS of the human CD20 gene, MS4A1. |