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The REEP5/TRAM1 complex binds SARS-CoV-2 NSP3 and promotes virus replication.
Li J, Gui Q, Liang FX, Sall J, Zhang Q, Duan Y, Dhabaria A, Askenazi M, Ueberheide B, Stapleford KA, Pagano M
J Virol. 2023 Sep 28:e0050723. doi: 10.1128/jvi.00507-23. PubMed Article

Plasmids from Article

ID Plasmid Purpose
210312NSP3C-FR-2StrepExpresses the C terminal of SARS-CoV-2 NSP3 in mammalian cells
210315NSP3-FR-2StrepExpresses SARS-CoV-2 NSP3 in mammalian cells
210316NSP4-FR-2StrepExpresses SARS-CoV-2 NSP4 in mammalian cells
210317NSP6-FR-2StrepExpresses SARS-CoV-2 NSP6 in mammalian cells
210318NSP3C-EGFPExpresses the C terminal of SARS-CoV-2 NSP3 fused with EGFP tag in mammalian cells
210319NSP4-mCherryExpresses SARS-CoV-2 NSP4 fused with mCherry in mammalian cells
210339NSP4-WT-FlagExpresses SARS-CoV-2 NSP4 fused with Flag in mammalian cells
210340NSP4-H120N/F121L-FlagExpresses SARS-CoV-2 NSP4-H120N/F121L fused with Flag in mammalian cells
210341NSP4-Δ220-234-FlagExpresses SARS-CoV-2 NSP4-Δ220-234 fused with Flag in mammalian cells
210342PcDNA3.1-FlagEmpty Vector for molecular cloning
210343PcDNA3.1-2StrepEmpty Vector for molecular cloning
210344NSP2-FlagExpresses SARS-CoV-2 NSP2 in mammalian cells
210345NSP6-FlagExpresses SARS-CoV-2 NSP6 fused with Flag in mammalian cells
210346NSP14-FlagExpresses SARS-CoV-2 NSP14 fused with Flag in mammalian cells
210347Flag-V0AExpresses ATP6V0A in mammalian cells
210348Flag-V0D1Expresses ATP6V0D1 in mammalian cells
210349NSP3C-FlagExpresses the C terminal of SARS-CoV-2 NSP3 fused with Flag in mammalian cells
210350REEP5-2StrepExpresses REEP5 fused with 2Strep in mammalian cells
210351REEP6-2StrepExpresses REEP6 fused with 2Strep in mammalian cells
210352TRAM1-2StrepExpresses TRAM1 fused with 2Strep in mammalian cells
210353SGTA-2StrepExpresses SGTA fused with 2Strep in mammalian cells
210354DNAJC11-2StrepExpresses DNAJC11 fused with 2Strep in mammalian cells
210355IDE-2StrepExpresses IDE fused with 2Strep in mammalian cells
210356XPO6-2StrepExpresses XPO6 fused with 2Strep in mammalian cells
210357FKBP10-2StrepExpresses FKBP10 fused with 2Strep in mammalian cells
210359ATG9A-2STrepExpresses ATG9A fused with 2Strep in mammalian cells
210360TMEM106B-2StrepExpresses TMEM106B fused with 2Strep in mammalian cells
210361STIM1-2StrepExpresses STIM1 fused with 2Strep in mammalian cells
210362GLG1-2StrepExpresses GLG1 fused with 2Strep in mammalian cells
210363REEP5-EGFPExpresses REEP5 fused with EGFP in mammalian cells
210364REEP6-EGFPExpresses REEP6 fused with EGFP in mammalian cells
210365TRAM1-EGFPExpresses TRAM1 fused with EGFP in mammalian cells
210366TMEM106B-EGFPExpresses TMEM106B fused with EGFP in mammalian cells
210367ATG9A-EGFPExpresses ATG9A fused with EGFP in mammalian cells
210368DNAJC11-EGFPExpresses DNAJC11 fused with EGFP in mammalian cells
210369FKBP10-EGFPExpresses FKBP10 fused with EGFP in mammalian cells
210370STIM1-EGFPExpresses STIM1 fused with EGFP in mammalian cells
210371XPO6-EGFPExpresses XPO6 fused with EGFP in mammalian cells
210372GLG1-EGFPExpresses GLG1 fused with EGFP in mammalian cells
210373SGTA-EGFPExpresses SGTA fused with EGFP in mammalian cells
210374TRAM2-2StrepExpresses TRAM2 fused with 2Strep in mammalian cells
210375SEC61B-2StrepExpresses SEC61B fused with 2Strep in mammalian cells
210376SARS-CoV-2 N-2StrepExpresses SARS-CoV-2 N protein fused with 2Strep in mammalian cells
210377NSP3C-mCherryExpresses the C terminal of SARS-CoV-2 NSP3 fused with mCherry tag in mammalian cells
210378NSP4-EGFPExpresses SARS-CoV-2 NSP4 fused with EGFP in mammalian cells

Antibodies from Article