NSP3-FR-2Strep
(Plasmid
#210315)
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PurposeExpresses SARS-CoV-2 NSP3 in mammalian cells
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Depositing Lab
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Depositing OrganizationNYU Grossman School of Medicine
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 210315 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 210315-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 210315-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonePcDNA3.1
- Backbone size w/o insert (bp) 6000
- Total vector size (bp) 11400
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNSP3
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Alt namePLPRO
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SpeciesSARS-CoV-2
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Insert Size (bp)6000
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GenBank ID
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Entrez GeneORF1ab (a.k.a. GU280_gp01)
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Tag
/ Fusion Protein
- 2Strep (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGHR
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byFritz Roth, Addgene plasmid #154402
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 210315-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 210315-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NSP3-FR-2Strep was a gift from Michele Pagano (Addgene plasmid # 210315 ; http://n2t.net/addgene:210315 ; RRID:Addgene_210315) -
For your References section:
The REEP5/TRAM1 complex binds SARS-CoV-2 NSP3 and promotes virus replication. Li J, Gui Q, Liang FX, Sall J, Zhang Q, Duan Y, Dhabaria A, Askenazi M, Ueberheide B, Stapleford KA, Pagano M. J Virol. 2023 Sep 28:e0050723. doi: 10.1128/jvi.00507-23. 10.1128/jvi.00507-23 PubMed 37768083
