Compact engineered human mechanosensitive transactivation modules enable potent and versatile synthetic transcriptional control.
Mahata B, Cabrera A, Brenner DA, Guerra-Resendez RS, Li J, Goell J, Wang K, Guo Y, Escobar M, Parthasarathy AK, Szadowski H, Bedford G, Reed DR, Kim S, Hilton IB
Nat Methods. 2023 Oct 9. doi: 10.1038/s41592-023-02036-1. PubMed Article
Mahata B, Cabrera A, Brenner DA, Guerra-Resendez RS, Li J, Goell J, Wang K, Guo Y, Escobar M, Parthasarathy AK, Szadowski H, Bedford G, Reed DR, Kim S, Hilton IB
Nat Methods. 2023 Oct 9. doi: 10.1038/s41592-023-02036-1. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 210703 | EFS-SpdCas9 | This plasmid express EFS promoter driven human codon optimized SpdCas9 |
| 210704 | MCP-MSN | This plasmid express EF1a promoter driven MCP-MSN transactivation module |
| 210705 | MCP-NMS | This plasmid express EF1a promoter driven MCP-NMS transactivation module |
| 210706 | MCP-eN3x9 | This plasmid express EF1a promoter driven MCP-eN3x9 transactivation module |
| 210707 | MCP-3x9aaTAD | This Plasmid express EF1a promoter driven MCP-3x 9aa transactivation module |
| 210708 | AAV-hSyn-SpdCas9-W3SL | This Plasmid express hSyn promoter driven SpdCas9 |
| 210709 | AAV-U6-Agrp-(mouse)-MS2gRNA-hSyn-MCP-MSN | This Plasmid express U6 promoter driven SpdCas9 specific gRNA and hSyn promoter driven MCP-MSN transactivation module |
| 210710 | AAV-AIO-M11-gRNA-EFS-NMS-SadCas9 | This Plasmid express M11 promoter driven SadCas9 specific gRNA and EFS promoter driven NMS transactivation module fused to SadCas9 |
| 210711 | U6-CjCas9-MS2gRNA-Backbone | This plasmid express MS2 loop containing Cj Cas9 gRNA |