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Addgene
RHINO directs MMEJ to repair DNA breaks in mitosis.
Brambati A, Sacco O, Porcella S, Heyza J, Kareh M, Schmidt JC, Sfeir A
Science. 2023 Aug 11;381(6658):653-660. doi: 10.1126/science.adh3694. Epub 2023 Jul 13.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
211535hLig4 guide1 in pX458 sgRNA-1 against Lig4
211536hLig4 guide2 in pX458sgRNA-2 against Lig4
211537pLenti-GFP-2A-Puro-gATRIP_AsgRNA-A against ATRIP
211538pLenti-GFP-2A-Puro-gATRIP_BsgRNA-B against ATRIP
211539pLenti-GFP-2A-Puro-gETAA1_AsgRNA-A against ETAA1
211540pLenti-GFP-2A-Puro-gETAA1_BsgRNA-B against ETAA1
211541pLenti-GFP-2A-Puro-gHUS1_AsgRNA-A against HUS1
211542pLenti-GFP-2A-Puro-gHUS1_BsgRNA-B against HUS1
211543pLenti-GFP-2A-Puro-gIL25_AsgRNA-A against IL25
211544pLenti-GFP-2A-Puro-gRAD1_AsgRNA-A against RAD1
211545pLenti-GFP-2A-Puro-gRAD1_BsgRNA-B against RAD1
211611pLenti-GFP-2A-Puro-gRAD9A_AsgRNA-A against RAD9
211612pLenti-GFP-2A-Puro-gRAD9A_BsgRNA-B against RAD9
211613pLenti-GFP-2A-Puro-gRHNO1_AsgRNA-A against Rhno
211614pLenti-GFP-2A-Puro-gRHNO1_BsgRNA-B against Rhno
211616pLenti-h.Rhno1 Plk1mut7A-C-PAMmut-Myc-DDK-P2A-PuroRhno1 Plk1mut7A
211617pLenti-h.Rhno1 Cdk1mut7A-C-Myc-P2A-PuroRhno1 Cdk1mut7A
211619pLenti-h.Rhno1 delta TOPBP1 (T202A)-C-Myc-P2A-PuroRhno1 delta TOPBP1 (T202A)
211620pLenti-h.Rhno1 S51A (A)-C-Myc-DDK-P2A-PuroRhno1 S51A
211621pLenti-h.Rhno1 T52A (B)-C-Myc-DDK-P2A-PuroRhno1 T52A
211622pLenti-h.Rhno1 S141A (C)-C-Myc-DDK-P2A-PuroRhno1 S141A
211623pLenti-h.Rhno1 S162A (D)-C-Myc-DDK-P2A-PuroRhno1 S162A
211624pLenti-h.Rhno1 S163A (E)-C-Myc-DDK-P2A-PuroRhno1 S163A
211625pLenti-h.Rhno1 S164A (F)-C-Myc-DDK-P2A-PuroRhno1 S164A
211626pLenti-h.Rhno1 S178A (G)-C-Myc-DDK-P2A-PuroRhno1 S178A
211627PX458 pSp Cas9 Rhino1 tagACas9 Rhino1 tagA
211628lenti-CHMP4B gRNA1sgRNA-1 against CHMP4B
211629lenti-CHMP4B gRNA2sgRNA-2 against CHMP4B
211630lenti-ERCC1 gRNA1sgRNA-1 against ERCC1
211631lenti-ERCC1 gRNA2sgRNA-2 against ERCC1
211632lenti-ERCC6L gRNA1sgRNA-1 against ERCC6L
211633lenti-ERCC6L gRNA2sgRNA-2 agsinst ERCC6L
211634lenti-ZWILCH gRNA1sgRNA-1 against ZWILCH
211635lenti-ZWILCH gRNA2sgRNA-2 against ZWILCH
211636gRNA-POLQ-HalosgRNA against POLQ
211637HDR-HaloPOLQhalo donor

Antibodies from Article