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Targeting the non-coding genome and temozolomide signature enables CRISPR-mediated glioma oncolysis.
Tan IL, Perez AR, Lew RJ, Sun X, Baldwin A, Zhu YK, Shah MM, Berger MS, Doudna JA, Fellmann C
Cell Rep. 2023 Nov 28;42(11):113339. doi: 10.1016/j.celrep.2023.113339. Epub 2023 Nov 2. PubMed Article

Plasmids from Article

ID Plasmid Purpose
211644pCF226_EFS-Cas9-P2A-PuroEFS-Cas9-P2A-Puro
211645pCF823_EFS-Cas9-P2A-PuroEFS-Cas9-P2A-Puro
211646pCF287_EFS-TiCas9-P2A-PuroEFS-TiCas9-P2A-Puro. Tamoxifen-inducible SpyCas9.
211647pCF820-recipient_U6-sgRNA-EF1a-mCherry2U6-sgRNA-EF1a-mCherry2
211648pCF820-sgNT-1_U6-sgRNA-EF1a-mCherry2U6-sgRNA-EF1a-mCherry2
211649pCF820-sgCIDE-1_U6-sgRNA-EF1a-mCherry2U6-sgRNA-EF1a-mCherry2
211650pCF820-sgCIDE-2_U6-sgRNA-EF1a-mCherry2U6-sgRNA-EF1a-mCherry2
211651pCF821-recipient_U6-sgRNA-EF1a-mNeonGreenU6-sgRNA-EF1a-mNeonGreen
211652pCF821-sgNT-1_U6-sgRNA-EF1a-mNeonGreenU6-sgRNA-EF1a-mNeonGreen
211653pCF821-sgNT-2_U6-sgRNA-EF1a-mNeonGreenU6-sgRNA-EF1a-mNeonGreen
211654pCF821-sgNT-3_U6-sgRNA-EF1a-mNeonGreenU6-sgRNA-EF1a-mNeonGreen
211655pCF821-sgOR2B6-1_U6-sgRNA-EF1a-mNeonGreenU6-sgRNA-EF1a-mNeonGreen
211656pCF821-sgOR2B6-2_U6-sgRNA-EF1a-mNeonGreenU6-sgRNA-EF1a-mNeonGreen
211657pCF821-sgOR2B6-3_U6-sgRNA-EF1a-mNeonGreenU6-sgRNA-EF1a-mNeonGreen
211658pCF821-sgRPA1-1_U6-sgRNA-EF1a-mNeonGreenU6-sgRNA-EF1a-mNeonGreen
211659pCF821-sgRPA1-2_U6-sgRNA-EF1a-mNeonGreenU6-sgRNA-EF1a-mNeonGreen
211660pCF821-sgRPA1-3_U6-sgRNA-EF1a-mNeonGreenU6-sgRNA-EF1a-mNeonGreen
211661pCF821-sgCIDE-1_U6-sgRNA-EF1a-mNeonGreenU6-sgRNA-EF1a-mNeonGreen
211662pCF821-sgCIDE-2_U6-sgRNA-EF1a-mNeonGreenU6-sgRNA-EF1a-mNeonGreen
211663pCF821-sgCIDE-3_U6-sgRNA-EF1a-mNeonGreenU6-sgRNA-EF1a-mNeonGreen
211664pCF821-sgCIDE-4_U6-sgRNA-EF1a-mNeonGreenU6-sgRNA-EF1a-mNeonGreen
211665pCF821-sgCIDE-5_U6-sgRNA-EF1a-mNeonGreenU6-sgRNA-EF1a-mNeonGreen
211666pCF821-sgCIDE-6_U6-sgRNA-EF1a-mNeonGreenU6-sgRNA-EF1a-mNeonGreen
211667pCF821-sgCIDE-7_U6-sgRNA-EF1a-mNeonGreenU6-sgRNA-EF1a-mNeonGreen
211668pCF821-sgCIDE-8_U6-sgRNA-EF1a-mNeonGreenU6-sgRNA-EF1a-mNeonGreen
211669pCF821-sgCIDE-9_U6-sgRNA-EF1a-mNeonGreenU6-sgRNA-EF1a-mNeonGreen
211670pCF821-sgCIDE-10_U6-sgRNA-EF1a-mNeonGreenU6-sgRNA-EF1a-mNeonGreen
211671pCF821-sgCIDE-21_U6-sgRNA-EF1a-mNeonGreenU6-sgRNA-EF1a-mNeonGreen
211672pCF821-sgCIDE-22_U6-sgRNA-EF1a-mNeonGreenU6-sgRNA-EF1a-mNeonGreen
211673pCF821-sgCIDE-23_U6-sgRNA-EF1a-mNeonGreenU6-sgRNA-EF1a-mNeonGreen
211674pCF821-sgCIDE-24_U6-sgRNA-EF1a-mNeonGreenU6-sgRNA-EF1a-mNeonGreen
211675pCF821-sgCIDE-31_U6-sgRNA-EF1a-mNeonGreenU6-sgRNA-EF1a-mNeonGreen
211676pCF821-sgCIDE-32_U6-sgRNA-EF1a-mNeonGreenU6-sgRNA-EF1a-mNeonGreen
211677pCF821-sgCIDE-33_U6-sgRNA-EF1a-mNeonGreenU6-sgRNA-EF1a-mNeonGreen
211678pCF821-sgCIDE-34_U6-sgRNA-EF1a-mNeonGreenU6-sgRNA-EF1a-mNeonGreen
211679pCF821-sgCIDE-35_U6-sgRNA-EF1a-mNeonGreenU6-sgRNA-EF1a-mNeonGreen
211680pCF821-sgCIDE-36_U6-sgRNA-EF1a-mNeonGreenU6-sgRNA-EF1a-mNeonGreen
211681pCF821-sgCIDE-41_U6-sgRNA-EF1a-mNeonGreenU6-sgRNA-EF1a-mNeonGreen
211682pCF821-sgCIDE-42_U6-sgRNA-EF1a-mNeonGreenU6-sgRNA-EF1a-mNeonGreen
211683pCF821-sgCIDE-43_U6-sgRNA-EF1a-mNeonGreenU6-sgRNA-EF1a-mNeonGreen
211684pCF821-sgCIDE-44_U6-sgRNA-EF1a-mNeonGreenU6-sgRNA-EF1a-mNeonGreen
211685pCF821-sgCIDE-45_U6-sgRNA-EF1a-mNeonGreenU6-sgRNA-EF1a-mNeonGreen
211686pCF821-sgCIDE-46_U6-sgRNA-EF1a-mNeonGreenU6-sgRNA-EF1a-mNeonGreen
211687pCF822-recipient_U6-sgRNA-EFS-Cas9-P2A-PuroU6-sgRNA-EFS-Cas9-P2A-Puro
211688pCF826-recipient_U6-sgRNA-EFS-Cas9-P2A-mCherry2U6-sgRNA-EFS-Cas9-P2A-mCherry2
211689pCF826-sgNT-1_U6-sgRNA-EFS-Cas9-P2A-mCherry2U6-sgRNA-EFS-Cas9-P2A-mCherry2
211690pCF826-sgCIDE-1_U6-sgRNA-EFS-Cas9-P2A-mCherry2U6-sgRNA-EFS-Cas9-P2A-mCherry2
211691pCF826-sgCIDE-2_U6-sgRNA-EFS-Cas9-P2A-mCherry2U6-sgRNA-EFS-Cas9-P2A-mCherry2
211692pCF827-recipient_U6-sgRNA-EFS-Cas9-P2A-mNeonGreenU6-sgRNA-EFS-Cas9-P2A-mNeonGreen
211693pCF827-sgNT-1_U6-sgRNA-EFS-Cas9-P2A-mNeonGreenU6-sgRNA-EFS-Cas9-P2A-mNeonGreen
211694pCF827-sgNT-2_U6-sgRNA-EFS-Cas9-P2A-mNeonGreenU6-sgRNA-EFS-Cas9-P2A-mNeonGreen
211695pCF827-sgNT-3_U6-sgRNA-EFS-Cas9-P2A-mNeonGreenU6-sgRNA-EFS-Cas9-P2A-mNeonGreen
211696pCF827-sgCIDE-1_U6-sgRNA-EFS-Cas9-P2A-mNeonGreenU6-sgRNA-EFS-Cas9-P2A-mNeonGreen
211697pCF827-sgCIDE-2_U6-sgRNA-EFS-Cas9-P2A-mNeonGreenU6-sgRNA-EFS-Cas9-P2A-mNeonGreen
211698pCF827-sgCIDE-3_U6-sgRNA-EFS-Cas9-P2A-mNeonGreenU6-sgRNA-EFS-Cas9-P2A-mNeonGreen

Antibodies from Article