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A modular dCas9-based recruitment platform for combinatorial epigenome editing.
Swain T, Pflueger C, Freytag S, Poppe D, Pflueger J, Nguyen TV, Li JK, Lister R
Nucleic Acids Res. 2023 Nov 24:gkad1108. doi: 10.1093/nar/gkad1108. PubMed Article

Plasmids from Article

ID Plasmid Purpose
211766LLP773_pLenti-6xHRBKET-sgRNAMultiplexed gRNA plasmid targeting six different gene promoters (PACC1-2, EPCAM-2, B2M-4, KL-3, RBM3-1, HINT1-1), with mCherry selection
211767LLP774_dCas9-Spy-Snoop-Sunx5-Avi-Tag-BFP (dCas9-SSSavi-BFP)dCas9 docking array with four tag domains (Spy, Snoop, aGCN4, Avi), with BFP selection
211768LLP775_αGCN4-p65HSF1-COSunTag counterpart binding domain, aGCN4, fused to transcriptional activator p65HSF1, with GFP selection
211769LLP776_SpyCatcher-p65HSF1-COSpyTag counterpart binding domain, SpyCatcher, fused to transcriptional activator p65HSF1, with GFP selection
211770LLP777_SnoopCatcher-p65HSF1-COSnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional activator p65HSF1, with GFP selection
211771LLP778_Traptavidin-p65HSF1-COAviTag counterpart binding domain, Traptavidin, fused to transcriptional activator p65HSF1, with sfGFP selection
211772LLP779_αGCN4-VP64-COSunTag counterpart binding domain, αGCN4, fused to transcriptional activator VP64, with GFP selection
211773LLP780_SpyCatcher-KRAB-COSpyTag counterpart binding domain, SpyCatcher, fused to transcriptional repressor KRAB, with GFP selection
211774LLP781_SnoopCatcher-KRAB-COSnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional repressor KRAB, with GFP selection
211775LLP782_Traptavidin-KRAB-COAviTag counterpart binding domain, Traptavidin, fused to transcriptional repressor KRAB, with GFP selection
211776LLP783_SpyCatcher-DNMT3A-COSpyTag counterpart binding domain, SpyCatcher, fused to DNA methyltransferase DNMT3A, with GFP selection
211777LLP784_SnoopCatcher-DNMT3A-COSnoopTag counterpart binding domain, SnoopCatcher, fused to DNA methyltransferase DNMT3A, with GFP selection
211778LLP785_Traptavidin-DNMT3A-COAviTag counterpart binding domain, Traptavidin, fused to DNA methyltransferase DNMT3A, with GFP selection
211779LLP786_SpyCatcher-EZH2-FL-COSpyTag counterpart binding domain, SpyCatcher, fused to polycomb repressive complex subunit EZH2, with GFP selection
211780LLP787_SnoopCatcher-EZH2-FL-COSnoopTag counterpart binding domain, SnoopCatcher, fused to polycomb repressive complex subunit EZH2, with GFP selection
211781LLP788_Traptavidin-EZH2-FL-COAviTag counterpart binding domain, Traptavidin, fused to polycomb repressive complex subunit EZH2, with GFP selection
211782LLP618_αGCN4-KRAB-COSunTag counterpart binding domain, aGCN4, fused to transcriptional repressor KRAB, with GFP selection
211783LLP789_αGCN4-DNMT3A-COSunTag counterpart binding domain, aGCN4, fused to DNA methyltransferase DNMT3A, with GFP selection
211784LLP790_αGCN4-EZH2-FL-COSunTag counterpart binding domain, aGCN4, fused to polycomb repressive complex subunit EZH2, with GFP selection
211785LLP617_αGCN4-mCherrySunTag counterpart binding domain, aGCN4, fused to catalytically inert mCherry control, with GFP selection
211786LLP791_PB_dCas9-SSSavi_BirAPiggyBac plasmid with dCas9 docking array with four tag domains (Spy, Snoop, aGCN4, Avi), and BirA, with Hygro selection

Antibodies from Article